Identification of a consensus DNA-binding site for the TCP domain transcription factor TCP2 and its important roles in the growth and development of Arabidopsis
Identification of a consensus DNA-binding site for the TCP domain transcription factor TCP2 and its important roles in the growth and development of Arabidopsis
复制标题
TCP结构域转录因子TCP2共有DNA结合位点的鉴定及其在拟南芥生长发育中的重要作用
DOI：
10.1007/s11033-021-06233-z
复制
发表时间：
2021-03-10
影响因子：
2.8
通讯作者：
Liu, Suchun
中科院分区：
文献类型：
--
作者：
He, Zhimin;Zhou, Xiaomei;Liu, Suchun
TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTOR 1（TCP）转录因子控制各种植物物种生长和发育的多个方面。然而，很少有报道通过它们的特异性结合位点直接靶向调控基因，进而揭示它们在植物中的功能。随机结合位点选择法（RBSS）鉴定了TCP 2的一个共有DNA结合位点基序。TCP 2识别的DNA含有G（G/T）GGNCC（A/C）基序，与其他TCP结构域蛋白结合的基序高度一致。因此，该基序被认为是TCP 2的特异性DNA结合位点。生物钟相关基因1（Circadian clock associated 1，CCA 1）和EARLY FLOWER 3（EARLY FLOWER 3，ELF 3）由于含有类似的TCP 2结合位点或核心结合位点GGNCC而被认为是潜在的靶基因，并被发现通过DNA结合受到TCP 2的正调控。表型分析结果表明，TCP 2的突变和过表达导致叶片形态发生变异，尤其是TCP 2、4和10的双突变或三突变。TCPs的突变导致开花延迟。最后，TCP 2被证明通过介导茉莉酸信号通路影响下胚轴伸长。这些结果为进一步研究TCP 2的靶基因和阐明TCP 2在植物生长发育中的重要作用奠定了基础。
TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTOR 1 (TCP) transcription factors control multiple aspects of growth and development in various plant species. However, few genes were reported to be directly targeted and regulated by them through their specific binding sites, and then uncover their functions in plants. A consensus DNA-binding site motif of TCP2 was identified by random binding site selection (RBSS). DNA recognized by TCP2 contained the motif G(G/T)GGNCC(A/C), which showed high consistency with motifs bound by other TCP domain proteins. Consequently, this motif was regarded as the specific DNA-binding sites of TCP2. Circadian clock associated 1 (CCA1) and EARLY FLOWERING 3 (ELF3) were subsequently considered as potential target genes owing to the containing of the similar TCP2 binding sites or core binding sites GGNCC and found to be positively regulated by TCP2 via DNA binding. Phenotype analysis results showed that mutation and over-expression of TCP2 resulted in variations in leaf morphogenesis, especially the double or triple mutations of TCP2, 4 and 10. Mutations in TCPs caused late flowering. Finally, TCP2 was shown to influence hypocotyl elongation by mediating the jasmonate signaling pathway. Overall, these results provide a basis for future studies aimed at distinguishing the target genes of TCP2 and elucidating the important roles of TCP2 in plant growth and development.