AGE-RELATED-CHANGES IN BRAIN MICROANATOMY - SENSITIVITY TO TREATMENT WITH THE DIHYDROPYRIDINE CALCIUM-CHANNEL BLOCKER DARODIPINE (PY-108-068)
AGE-RELATED-CHANGES IN BRAIN MICROANATOMY - SENSITIVITY TO TREATMENT WITH THE DIHYDROPYRIDINE CALCIUM-CHANNEL BLOCKER DARODIPINE (PY-108-068)
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DOI：
10.1016/0361-9230(94)00210-r
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发表时间：
1995-01-01
影响因子：
3.8
通讯作者：
ZENG, YC
中科院分区：
文献类型：
--
作者：
AMENTA, F;CAVALLOTTI, D;ZENG, YC
用18~24月龄雄性Wistar大鼠灌胃给予达洛地平5 mg/kg/d，观察衰老及二氢吡啶钙拮抗剂达洛地平(PY 108-068)治疗对大鼠脑微解剖结构的影响。以12月龄未处理大鼠为成年参照组。与12月龄相比，24月龄大鼠的额叶和枕叶皮质、海马区和小脑皮层的神经细胞数量和碱性磷酸酶阳性毛细血管数量减少，脂褐素沉积增加。与年龄匹配的未治疗大鼠相比，达洛地平治疗组大鼠枕叶皮质和海马区的神经细胞数量较多，但额叶皮质和小脑皮质的神经细胞数量没有增加。在研究的所有大脑区域，脂褐素的沉积都减少了。达洛地平治疗的老年大鼠的额叶和枕叶皮质以及海马区碱性磷酸酶反应性毛细血管密度也增加。上述结果表明，达洛地平治疗能够对抗老年大鼠脑内发生的一些微解剖变化，而且不仅涉及微血管参数。枕叶(视觉)皮质和海马区是对达洛地平治疗更敏感的大脑区域。对这些发现的可能相关性进行了讨论。
The influence of aging and of treatment with the dihydropyridine Ca2+ antagonist darodipine (PY 108-068) on the age-related microanatomical changes of rat brain were studied in male Wistar rats treated from the 18th to the 24th month of age with an oral dose of 5 mg/kg/day of darodipine. Twelve-month-old untreated rats were used as an adult reference group. A decreased number of nerve cells and of alkaline phosphatase-positive capillaries and an increased lipofuscin deposition were observed in the frontal and occipital cortex, in the hippocampus, and in the cerebellar cortex of rats of 24 months in comparison with 12-month-old animals. The number of nerve cells was higher in the occipital cortex and in the hippocampus, but not in the frontal cortex and in the cerebellar cortex, of darodipine-treated rats in comparison with age-matched untreated animals. Lipofuscin deposition is reduced in all the brain areas investigated. The density of alkaline phosphatase-reactive capillaries is also increased in the frontal and occipital cortex and in the hippocampus of aged rats treated with darodipine. The above results suggest that treatment with darodipine is able to counter some microanatomical changes occurring in the brain of aged rats and involving not only microvascular parameters. The occipital (visual) cortex and the hippocampus were the cerebral areas more sensitive to treatment with darodipine. The possible relevance of these findings is discussed.