Surface proteins that promote adherence of Staphylococcus aureus to human desquamated nasal epithelial cells.
Surface proteins that promote adherence of Staphylococcus aureus to human desquamated nasal epithelial cells.
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DOI：
10.1186/1471-2180-9-22
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发表时间：
2009-01-30
期刊：
影响因子：
4.2
通讯作者：
Foster, Timothy J.
中科院分区：
文献类型：
--
作者：
Corrigan, Rebecca M.;Miajlovic, Helen;Foster, Timothy J.
金黄色葡萄球菌的自然栖息地是前鼻孔的湿润鳞状上皮。大约20%的人类携带S。金黄色葡萄球菌永久性地存在于鼻子中，另外60%的个体是间歇性携带者。S.金黄色葡萄球菌定植鼻上皮的部分原因是表面蛋白凝集因子B（Clf B）和铁调节的表面决定簇A（IsdA）的表达，其促进与存在于鼻前庭前部的脱落上皮细胞的粘附。S. IsdA和ClfB缺陷的金黄色葡萄球菌菌株纽曼表现出对鳞状细胞的粘附减少，但不是完全缺陷的，这表明其他细胞表面成分也可能起作用。研究了表面蛋白IsdA、ClfB和丝氨酸-天冬氨酸重复蛋白SdrC、SdrD和SdrE对S.金黄色葡萄球菌对脱屑的鼻上皮细胞。这是通过在替代革兰氏阳性宿主乳酸乳球菌表面表达ClfB、IsdA、SdrC、SdrD和SdrE以及分离S.金黄色葡萄球菌纽曼缺陷的一个或多个因素。测量菌株对从志愿者鼻孔分离的鳞状细胞的粘附水平。结果一致表明，ClfB、IsdA、SdrC和SdrD均对S.金黄色葡萄球菌粘附于鳞状细胞。缺乏所有四种蛋白质的突变体在粘附上完全有缺陷。S.金黄色葡萄球菌纽曼粘附于脱落的鼻上皮细胞是多因素的，涉及SdrD和SdrC以及ClfB和IsdA。
The natural habitat of Staphylococcus aureus is the moist squamous epithelium in the anterior nares. About 20% of the human population carry S. aureus permanently in their noses and another 60% of individuals are intermittent carriers. The ability of S. aureus to colonize the nasal epithelium is in part due to expression of surface proteins clumping factor B (ClfB) and the iron-regulated surface determinant A (IsdA), which promote adhesion to desquamated epithelial cells present in the anterior part of the nasal vestibule. S. aureus strain Newman defective in IsdA and ClfB exhibited reduced but not completely defective adherence to squamous cells in indicating that other cell surface components might also contribute. Surface proteins IsdA, ClfB, and the serine-aspartic acid repeat proteins SdrC, SdrD and SdrE were investigated to determine their contribution to the adherence of S. aureus to desquamated nasal epithelial cells. This was achieved by expression of ClfB, IsdA, SdrC, SdrD and SdrE on the surface of the surrogate Gram-positive host Lactococcus lactis and by isolating mutants of S. aureus Newman defective in one or more factor. The level of adherence of strains to squamous cells isolated from the nares of volunteers was measured. Results consistently showed that ClfB, IsdA, SdrC and SdrD each contributed to the ability of S. aureus to adhere to squamous cells. A mutant lacking all four proteins was completely defective in adherence. The ability of S. aureus Newman to adhere to desquamated nasal epithelial cells is multifactorial and involves SdrD and SdrC as well as ClfB and IsdA.
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影响因子：
3.1
作者：
Clarke, Simon R.;Foster, Simon J.
通讯作者：
Foster, Simon J.
DOI：
10.1007/bf00399503
发表时间：
1983-01-01
期刊：
ANTONIE VAN LEEUWENHOEK JOURNAL OF MICROBIOLOGY
影响因子：
--
作者：
GASSON, MJ
通讯作者：
GASSON, MJ
影响因子：
3.6
作者：
Fitzgerald, JR;Loughman, A;Foster, TJ
通讯作者：
Foster, TJ
影响因子：
4.8
作者：
McAleese, FM;Walsh, EJ;Foster, TJ
通讯作者：
Foster, TJ
影响因子：
3.6
作者：
Clarke, SR;Wiltshire, MD;Foster, SJ
通讯作者：
Foster, SJ