Wing patterning genes of Nilaparvata lugens identification by transcriptome analysis, and their differential expression profile in wing pads between brachypterous and macropterous morphs
Wing patterning genes of Nilaparvata lugens identification by transcriptome analysis, and their differential expression profile in wing pads between brachypterous and macropterous morphs
复制标题
通过转录组分析鉴定褐飞虱翅膀图案基因及其在短翅类和大翅类翅垫中的差异表达谱
DOI：
10.1016/s2095-3119(14)60948-5
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发表时间：
2015-01-01
影响因子：
4.8
通讯作者：
Hua Hong-xia
中科院分区：
文献类型：
--
作者：
Li Kai-yin;Hu Ding-bang;Hua Hong-xia
褐飞虱是水稻上的重要害虫。这一物种产生的macropterous和bracyypterous变种响应环境线索，这使得它非常难以控制。本文研究了黑翅夜蛾翅型形成的分子基础。卢根斯仍然是未知的。因此，有必要对黑翅夜蛾的翅型基因进行鉴定。lugens，并阐明翅型基因在长翅型和短翅型之间的表达差异。高通量深度测序N. Lugens翼垫产生116744580个原始读数和113042700个干净读数。所有的读段被组装成55 963个单基因，平均长度为804 bp。当E值截止值为1.0E（-5 ° C）时，在NCBI-NR（NCBI非冗余蛋白质序列）和NCBI-NT（NCBI核苷酸序列）数据库中分别有18 359和2 883个unigens匹配。将16 502个unigenes归入GO（gene ontology）分类，9 709个ungenes归入26个COG（cluster of orthopathic groups of proteins）分类，6 724个unigenes归入不同的KEGG（京都基因和基因组百科全书）途径。共鉴定出56个与黑腹果蝇或赤拟谷盗的翅型基因同源的unigene。从56个unigenes中选择了24个unigenes，并通过qRT-PCR检测了它们在大翅型品系和短翅型品系之间五个若虫阶段的表达水平。双因素方差分析表明，发育阶段对所有24个基因的表达水平均有显著影响（P
The brown planthopper, Nilaparvata lugens is an economically important pest on rice plants. This species produces macropterous and brachypterous morphs in response to environmental cues, which makes it very difficult to control. The molecular basis of wing patterning in N. lugens is still unknown. It is necessary to identify wing patterning genes of N. lugens, and also to clarify the expression differences of wing patterning genes between macropterous and brachypterous morphs. High-throughput deep sequencing of transcriptome of N. lugens wing pad yielded 116 744 580 raw reads and 113 042 700 clean reads. All the reads were assembled into 55 963 unigenes with an average length of 804 bp. With the E-value cut-off of 1.0E(-5),18 359 and 2 883 unigens had hits in NCBI-NR (NCBI non-redundant protein sequences) and NCBI-NT (NCBI nucleotide sequences) databases, respectively. A total of 16 502 unigenes were assigned to GO (gene ontology) classification, 9 709 ungenes were grouped into 26 COG (cluster of orthologous groups of proteins) classifications, and 6 724 unigenes were assigned to different KEGG (Kyoto encyclopedia of genes and genomes) pathways. In total, 56 unigenes which are homologous to wing patterning genes of Drosophila melanogaster or Tribolium castaneum were identified. Out of the 56 unigenes, 24 unigenes were selected, and their expression levels across the five nymphal stages between macropterous strain and brachypterous strain were examined by qRT-PCR. Two-way ANOVA analysis showed that development stage had significant effects on the expression level of all the 24 genes (P