Mass sensors with mechanical traps for weighing single cells in different fluids.
Mass sensors with mechanical traps for weighing single cells in different fluids.
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DOI：
10.1039/c1lc20736a
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发表时间：
2011-11
期刊：
影响因子：
6.1
通讯作者：
Yaochung Weng;Francisco Feijó Delgado;S. Son;T. Burg;S. Wasserman;S. Manalis
中科院分区：
文献类型：
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作者：
Yaochung Weng;Francisco Feijó Delgado;S. Son;T. Burg;S. Wasserman;S. Manalis
我们提出了两种方法，通过这种方法，单个细胞可以在悬浮微通道谐振器（SMR）质量传感器中被机械捕获和连续监测。由于被困细胞周围的液体可以根据需要快速和完全替换，因此我们的方法非常适合测量细胞大小和生长对药物或其他化学刺激的反应。我们通过测量单个聚苯乙烯珠和酿酒酵母细胞的密度来验证我们的方法，精度约为10(-3)g cm(-3)，并通过监测药物治疗前后单个小鼠淋巴母细胞的生长情况。
We present two methods by which single cells can be mechanically trapped and continuously monitored within the suspended microchannel resonator (SMR) mass sensor. Since the fluid surrounding the trapped cell can be quickly and completely replaced on demand, our methods are well suited for measuring changes in cell size and growth in response to drugs or other chemical stimuli. We validate our methods by measuring the density of single polystyrene beads and Saccharomyces cerevisiae yeast cells with a precision of approximately 10(-3) g cm(-3), and by monitoring the growth of single mouse lymphoblast cells before and after drug treatment.