Adeno-associated virus vector integration.
Adeno-associated virus vector integration.
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发表时间：
2009-08
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通讯作者：
D. Deyle;D. Russell
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作者：
D. Deyle;D. Russell
腺相关病毒（AAV）载体有效地转染各种细胞类型，并且可以在体内产生转基因的长期表达。虽然AAV载体基因组可以作为附加体在细胞内持续存在，但在各种实验环境中观察到载体整合，无论是在可能发生DNA损伤的非同源位点还是通过同源重组。在一些情况下，整合对于AAV载体的治疗或实验功效是必需的。最近，由AAV载体的整合引起的插入诱变与小鼠中的肿瘤发生相关，这一考虑可能与某些临床应用相关。
Adeno-associated virus (AAV) vectors efficiently transduce various cell types and can produce long-term expression of transgenes in vivo. Although AAV vector genomes can persist within cells as episomes, vector integration has been observed in various experimental settings, either at non-homologous sites where DNA damage may have occurred or by homologous recombination. In some cases, integration is essential for the therapeutic or experimental efficacy of AAV vectors. Recently, insertional mutagenesis resulting from the integration of AAV vectors was associated with tumorigenesis in mice, a consideration that may have relevance for certain clinical applications.