BMP2-coprecipitated calcium phosphate granules enhance osteoinductivity of deproteinized bovine bone, and bone formation during critical-sized bone defect healing.
BMP2-coprecipitated calcium phosphate granules enhance osteoinductivity of deproteinized bovine bone, and bone formation during critical-sized bone defect healing.
复制标题
BMP2共沉淀磷酸钙颗粒增强脱蛋白牛骨的骨诱导性以及临界尺寸骨缺损愈合过程中的骨形成
DOI：
10.1038/srep41800
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发表时间：
2017-01-31
影响因子：
4.6
通讯作者：
Liu Y
中科院分区：
文献类型：
--
作者：
Liu T;Zheng Y;Wu G;Wismeijer D;Pathak JL;Liu Y
临床上用于填充临界尺寸骨缺损（CSBD）的材料，如脱蛋白牛骨（DBB），大多缺乏骨诱导性，其治疗效果远不能令人满意。骨形态发生蛋白2（BMP 2）-共沉淀仿生磷酸钙颗粒（BMP 2-cop.BioCaP）在CSBD愈合过程中对DBB移植物骨诱导的作用仍然未知。我们研究了BMP 2-cop.BioCaP是否影响DBB的骨诱导性、骨形成和CSBD愈合过程中的异物反应。将DBB + BMP 2-cop、BioCaP、DBB、DBB + BMP 2、DBB + BioCaP和自体骨移植物植入绵羊的CSBD中。在第4周和第8周对骨形成、DBB/BioCaP降解性、异物反应和DBB的骨诱导性进行组织学和组织形态计量学分析。BMP 2-cop、BioCaP和DBB的组合与自体骨移植一样有效地治愈CSBD。在DBB + BMP 2-cop. BioCaP-组中，约95%的BMP 2-cop.BioCaP在第8周已被降解并被新骨取代。与其他组相比，DBB + BMP 2-cop. BioCaP-组的异物反应减少。单独使用BMP 2-cop.BioCaP未实现令人满意的骨修复。结论：BMP 2-cop.BioCaP具有良好的降解性和生物相容性，并能增强DBB在绵羊CSBD愈合过程中的骨诱导活性，提示BMP 2-cop.BioCaP可作为一种潜在的骨诱导剂，用于增强移植材料的临床治疗效果。
Most materials used clinically for filling critical-sized bone defects (CSBD), such as deproteinized bovine bone (DBB), lack osteoinductivity so that their therapeutic effects are far from satisfactory. The effect of bone morphogenic protein 2 (BMP2)-coprecipitated biomimetic calcium phosphate granules (BMP2-cop.BioCaP) on osteoinduction of DBB graft(s) during CSBD healing is still unknown. We investigated whether BMP2-cop.BioCaP affects the osteoinductivity of DBB, bone formation, and foreign body reaction during CSBD healing. DBB + BMP2-cop.BioCaP, DBB, DBB + BMP2, DBB + BioCaP, and autologous bone grafts were implanted in the CSBD of sheep. Bone formation, DBB/BioCaP degradability, foreign body reaction, and osteoinductivity of DBB were analyzed histologically and histomorphometrically at week 4 and 8. Combination of BMP2-cop.BioCaP and DBB healed CSBD as effectively as autologous bone grafts. About 95% of the BMP2-cop.BioCaP had been degraded and replaced by new bone at week 8 in the DBB + BMP2-cop.BioCaP-group. Foreign body reaction was reduced in the DBB + BMP2-cop.BioCaP-group compared to the other groups. The independent use of the BMP2-cop.BioCaP did not achieve a satisfactory bone repair. In conclusion, the BMP2-cop.BioCaP showed good degradability and biocompatibility, and enhanced osteoinductivity of DBB during CSBD healing in sheep, suggesting BMP2-cop.BioCaP as a potential osteoinducer to enhance the therapeutic effects of the graft materials in clinic.