Histone H3 Lysine 9 Methyltransferase DIM5 Is Required for the Development and Virulence of Botrytis cinerea.
Histone H3 Lysine 9 Methyltransferase DIM5 Is Required for the Development and Virulence of Botrytis cinerea.
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DOI：
10.3389/fmicb.2016.01289
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发表时间：
2016
影响因子：
5.2
通讯作者：
Chen T
中科院分区：
文献类型：
--
作者：
Zhang X;Liu X;Zhao Y;Cheng J;Xie J;Fu Y;Jiang D;Chen T
组蛋白甲基化广泛存在于动物、植物和真菌中，组蛋白H3的甲基化修饰具有重要的生物学功能。组蛋白H3(H3K9)的Lys9甲基化已被证明调节染色质结构、基因沉默、转录激活、植物代谢等过程。在这项工作中，我们研究了灰霉病菌H3K9甲基转移酶基因BcDIM5的功能，该基因包含一个预设结构域、一个设定结构域和一个后置结构域。对BcDIM5基因敲除转化子的鉴定表明，BcDIM5基因敲除转化子的菌丝生长速度和分生孢子和菌核产量显著降低，而互补转化可以使表型恢复到野生型的水平。致病力测定表明，BcDIM5对灰霉病菌的完全致病力是必需的。BcDIM5基因敲除转化子的毒力降低，部分致病基因表达下调，H3K9三甲基化水平显著降低。然而，另外两个基因异染色质蛋白1(HP1)BcHP1和DNA甲基转移酶(DIM2)BcDIM2的敲除转化子与野生型相比，在生长表型和毒力方面没有明显的变化。我们的结果表明，H3K9甲基转移酶BcDIM5是H3K9三甲基化所必需的，以调节灰霉病菌的发育和毒力。
Histone methylation is widely present in animals, plants and fungi, and the methylation modification of histone H3 has important biological functions. Methylation of Lys9 of histone H3 (H3K9) has been proven to regulate chromatin structure, gene silencing, transcriptional activation, plant metabolism, and other processes. In this work, we investigated the functions of a H3K9 methyltransferase gene BcDIM5 in Botrytis cinerea, which contains a PreSET domain, a SET domain and a PostSET domain. Characterization of BcDIM5 knockout transformants showed that the hyphal growth rate and production of conidiophores and sclerotia were significantly reduced, while complementary transformation of BcDIM5 could restore the phenotypes to the levels of wild type. Pathogenicity assays revealed that BcDIM5 was essential for full virulence of B. cinerea. BcDIM5 knockout transformants exhibited decreased virulence, down-regulated expression of some pathogenic genes and drastically decreased H3K9 trimethylation level. However, knockout transformants of other two genes heterochromatin protein 1 (HP1) BcHP1 and DNA methyltransferase (DIM2) BcDIM2 did not exhibit significant change in the growth phenotype and virulence compared with the wild type. Our results indicate that H3K9 methyltransferase BcDIM5 is required for H3K9 trimethylation to regulate the development and virulence of B. cinerea.