An insect model for assessing oxidative stress related to arsenic toxicity.
An insect model for assessing oxidative stress related to arsenic toxicity.
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用于评估与砷毒性相关的氧化应激的昆虫模型。
DOI：
10.1002/arch.940290209
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Pardini,RS
中科院分区：
文献类型：
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作者：
Zaman,K;MacGill,RS;Johnson,JE;Ahmad,S;Pardini,RS
用三价砷(As3+，NaAsO2)和五价砷(As5+，Na2HAsO4)对成虫、家蝇和四龄甘蓝轮虫的氧化应激反应进行了研究。家蝇对As3+和As5+的LC_(50)分别为0.008和0.011%w/v，48h后对这两种形式的砷都高度敏感。尼拉尔瓦按蚊对饲料As_(3+)的LC_(50)为0.032%w/w，但对As_(5+)的耐受性较好，暴露48 h后的LC_(50)为0.794%。As3+和As5+的最小急性致死剂量差异很大，但两种昆虫的平均致死剂量均为0.005%。评估了这两种价态的砷在自由接触到大约LC5水平的昆虫中诱导氧化应激的潜力。观察超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽转移酶(GST)、谷胱甘肽转移酶(GSTPx)、谷胱甘肽还原酶(GR)等抗氧化酶活性的变化，以及脂质过氧化和蛋白质氧化的增加。As3+INM诱导的SOD(1.3倍)、GST(1.6倍)和GR(1.5倍)。家猫和GSTPX未受影响。As~(5+)对M_2无影响。家属品。内部。As~(3+)对抗氧化酶活性无明显影响，但对SOD的抑制率为29.4%，对GST的抑制率为1.4倍。As~(5+)除对SOD的抑制作用达41.2%外，其余作用不明显。脂质过氧化和蛋白质氧化代表了更强的氧化应激指标，在这两种昆虫中，这两种昆虫的水平都提高了2.9倍。然而，基于抗氧化酶对砷阴离子的反应，砷诱导的氧化应激在两种昆虫中的作用方式可能不同。在这方面得到进一步澄清之前，目前的证据支持As3+作为一种促氧化剂的前景，特别是形式。家属品。©1995 Wiley-Liss公司
The potential usefulness of an insect model to evaluate oxidative stress induced by environmental pollutants was examined with trivalent arsenic (As3+, NaAsO2) and pentavalent arsenic (As5+, Na2HAsO4) in adult female house flies,Musca domestica, and fourth‐instar cabbage loopers,Trichoplusia ni. M. domesticawas highly susceptible to both forms of arsenic following 48 h exposure in the drinking water with LC50s of 0.008 and 0.011% w/v for As3+and As5+, respectively.T. nilarvae were susceptible to dietary As3+with an LC50of 0.032% w/w but seem to tolerate As5+well with an LC50of 0.794% concentration after 48 h exposure. The minimally acute LC5dose of both As3+and As5+varied considerably but averaged 0.005% for both insects. The potential of both valencies of arsenic for inducing oxidative stress in the insects exposed ad libitum to approximately LC5levels was assessed. The parameters examined were the alterations of the antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione transferase (GST), the peroxidase activity of glutathione transferase (GSTPX), and glutathione reductase (GR), and increases in lipid peroxidation and protein oxidation. SOD (1.3‐fold), GST (1.6‐fold), and GR (1.5‐fold) were induced by As3+inM. domesticabut CAT and GSTPX were not affected. As5+had no effect onM. domestica. InT. ni, the antioxidant enzyme activities were not affected by As3+except for SOD which was suppressed by 29.4% and GST which was induced by 1.4‐fold. As5+had no effect except the suppression of SOD by 41.2%. Lipid peroxidation and protein oxidation, which represent stronger indices of oxidative stress, were elevated in both insects by up to 2.9‐fold. However, based on the antioxidant enzyme response to the arsenic anions, the mode of action of arsenic induced oxidative stress may differ between the two insects. Until this aspect is further clarified, evidence at this time favors the prospect of As3+as a pro‐oxidant, especially forM. domestica. © 1995 Wiley‐Liss, Inc.