INDUCTION OF APOPTOTIC DNA FRAGMENTATION AND CELL-DEATH BY NATURAL CERAMIDE
INDUCTION OF APOPTOTIC DNA FRAGMENTATION AND CELL-DEATH BY NATURAL CERAMIDE
复制标题
DOI：
10.1016/0014-5793(94)01428-4
复制
发表时间：
1995-01-23
期刊：
影响因子：
3.5
通讯作者：
HIRABAYASHI, Y
中科院分区：
文献类型：
--
作者：
JI, L;ZHANG, G;HIRABAYASHI, Y
我们发现乙醇和十二烷的溶剂混合物能够将天然神经酰胺（Cer）分散到水溶液中。在U937细胞中，向培养基中加入天然Cer可引起DNA片段化的特征性模式，这与TNF α引起的DNA片段化模式难以区分。Cer的有效浓度低至25 nM。本研究提供了直接证据，证明天然Cer作为第二信使介导TNF α诱导的DNA断裂。利用这种溶剂将疏水性天然Cer传递到细胞中，有助于阐明Cer的生物学功能。
We have found that a solvent mixture of ethanol and dodecane was able to disperse natural ceramide (Cer) into aqueous solution. In U937 cells, addition of natural Cer to medium caused a characteristic pattern of DNA fragmentation, which is indistinguishable from that caused by TNF alpha. The effective concentration of Cer is as low as 25 nM. The present study provides direct evidence that natural Cer functions as a second messenger mediating TNF alpha-induced DNA fragmentation. The use of this solvent to deliver hydrophobic natural Cer to cells mill contribute to the elucidation of the biological function of Cer.