Depth-dependent biomechanical and biochemical properties of fetal, newborn, and tissue-engineered articular cartilage
Depth-dependent biomechanical and biochemical properties of fetal, newborn, and tissue-engineered articular cartilage
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DOI：
10.1016/j.jbiomech.2005.11.002
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发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Sah, Robert L.
中科院分区：
文献类型：
--
作者：
Klein, Travis J.;Chaudhry, Manu;Sah, Robert L.
成人关节软骨具有深度依赖性的机械和生化特性，有助于区域特异性功能。已知未成熟软骨和组织工程软骨的压缩模量低于成人软骨的压缩模量。本研究的目的是确定这种组织是否表现出深度依赖性的压缩性能，以及这些深度变化的性能如何与组织的细胞和基质成分相关。胎儿和新生牛关节软骨的压缩模量随深度增加（p < 0.05），从顶部0.1mm（分别为28 +/- 13 kPa，141 +/- 10 kPa）到组织中1 mm深处增加4-5倍。同样，糖胺聚糖和胶原含量随深度增加（均p < 0.001），并与模量相关（均p < 0.01）。相比之下，通过分层或混合来自关节软骨的表层和中间区的细胞形成的组织工程化软骨在两个结构表面处表现出类似的柔软区域，如大的平衡应变所例示的。未成熟软骨的特性为组织工程软骨的开发提供了一个模板，其目的是通过再现软骨的自然发育和生长过程来修复软骨缺损。这些结果表明，虽然深度依赖性的特性可能是重要的工程到软骨结构，问题以外的细胞异质性必须得到解决，以产生这样的组织。(c)2005爱思唯尔有限公司保留所有权利。
Adult articular cartilage has depth-dependent mechanical and biochemical properties which contribute to zone-specific functions. The compressive moduli of immature cartilage and tissue-engineered cartilage are known to be lower than those of adult cartilage. The objective of this study was to determine if such tissues exhibit depth-dependent compressive properties, and how these depth-varying properties were correlated with cell and matrix composition of the tissue. The compressive moduli of fetal and newborn bovine articular cartilage increased with depth (p < 0.05) by a factor of 4-5 from the top 0.1 mm (28 +/- 13 kPa, 141 +/- 10 kPa, respectively) to 1 mm deep into the tissue. Likewise, the glycosaminoglycan and collagen content increased with depth (both p < 0.001), and correlated with the modulus (both p < 0.01). In contrast, tissue-engineered cartilage formed by either layering or mixing cells from the superficial and middle zone of articular cartilage exhibited similarly soft regions at both construct surfaces, as exemplified by large equilibrium strains. The properties of immature cartilage may provide a template for developing tissue-engineered cartilage which aims to repair cartilage defects by recapitulating the natural development and growth processes. These results suggest that while depth-dependent properties may be important to engineer into cartilage constructs, issues other than cell heterogeneity must be addressed to generate such tissues. (c) 2005 Elsevier Ltd. All rights reserved.