Highly sensitive diagnosis of amyloid and various amyloid syndromes using Congo red fluorescence
Highly sensitive diagnosis of amyloid and various amyloid syndromes using Congo red fluorescence
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DOI：
10.1007/s004280050471
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发表时间：
2000-05-01
期刊：
影响因子：
3.5
通讯作者：
Linke, RP
中科院分区：
文献类型：
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作者：
Linke, RP
为了找到如何最好地尽早诊断淀粉样蛋白沉积，已比较了可应用于诊断组织切片中淀粉样蛋白的三种不同方法的敏感性：刚果红染色法（CR）、CR和免疫细胞化学联合法（CRIC）和刚果红荧光法（CRF）。组织块来自25名患者，包括11名化学上不同的淀粉样蛋白疾病和3名化学上不确定的淀粉样蛋白疾病。结果表明：（a）CRF比CR或CRIC更敏感，如定性和定量所示，（B）CRF因此可用于追踪其他两种方法可能遗漏的甚至微小的淀粉样蛋白沉积物;（c）CRF和CRIC的特异性通过CRF标记和IC标记区域的绿色双折射（GB）的证明而在双染色切片上得到保证;（d）CRF可以通过仅改变光源在现场进行;和（e）CRF不受全等IC色原覆盖物的阻碍，这确保了淀粉样蛋白沉积物的特定分类适用于不同的淀粉样蛋白类别。总之，CRF被证明是直接诊断组织切片中淀粉样蛋白的最敏感方法。因此，这种方法可以允许淀粉样蛋白的最早诊断和分类，这是淀粉样蛋白类别特异性治疗的良好基础，同时器官损伤仍然最小。
In order to find how best to diagnose amyloid deposits as early as possible, the sensitivity of three different methods that can be applied to the diagnosis of amyloid in tissue sections have been compared: the Congo red staining method (CR), the combination of CR and immunocytochemistry (CRIC) and Congo red fluorescence (CRF). Tissue blocks were available from 25 patients, including 11 with immunohistochemically distinct and 3 with chemically undefined amyloid diseases. The results revealed (a) that CRF is more sensitive than either CR or CRIC, as shown qualitatively and quantitatively, (b) that CRF can therefore be utilized to track down even minute amyloid deposits, which can be missed by the other two methods; (c) that the specificity of CRF and CRIC is secured on double-stained sections by the demonstration of green birefringence (GB) of the CRF-marked and IC-marked areas; (d) that CRF can be performed on the spot by just changing the light source; and (e) that CRF is ndt hampered by the congruent IC chromogen overlay, which ensures the specific classification of the amyloid deposits as applied to different amyloid classes. In conclusion, CRF was demonstrated to be the most sensitive method for direct diagnosis of amyloid in tissue sections. This method can, therefore, allow the earliest diagnosis and classification of amyloid, which is a good basis for an amyloid class-specific therapy while organ damage is still minimal.