Analysis of the macular pigment by HPLC: retinal distribution and age study.
Analysis of the macular pigment by HPLC: retinal distribution and age study.
复制标题
DOI：
--
复制
发表时间：
1988-06
影响因子：
4.4
通讯作者：
R. A. Done;J. Landrum;Fernandez;Sara L. Tarsis
中科院分区：
文献类型：
--
作者：
R. A. Done;J. Landrum;Fernandez;Sara L. Tarsis
高效液相色谱法（HPCL）已被用来研究整个人视网膜的玉米黄质和叶黄素，黄斑色素的两个主要成分的分布。还研究了个人之间的差异，以揭示可能与年龄有关的影响。这两种色素在产前眼睛（妊娠约20周）中检测到，但没有形成可见的黄色斑点。一般来说，它们在出生后6个月左右才容易辨别。对于87名年龄在3岁至95岁之间的捐赠者，没有观察到任何色素的量与年龄的依赖性。其中约90%，玉米黄质占主导地位。对于剩下的10%，以及7名最年轻的捐赠者（均在2岁以下），以及产前眼睛中，叶黄素是主要色素。在单个视网膜中，叶黄素：玉米黄质比率从中心0-0.25 mm中的平均约1：2.4增加到周边（8.7-12.2 mm）中的超过2：1。该比率随偏心率的变化与相应的杆锥比呈线性相关。一个选择性的摄取机制，这导致锥和杆优先获得玉米黄质和叶黄素，分别可以解释这种相关性。
High performance liquid chromatography (HPCL) has been employed to study the distribution throughout the human retina of zeaxanthin and lutein, the two major components of the macular pigment. Differences between individuals have also been studied with a view to uncovering possible age-related effects. Both pigments were detected in prenatal eyes (approximately 20 weeks gestation) but did not form a visible yellow spot. Generally they were not easily discernible until about 6 months after birth. For 87 donors between the ages of 3 and 95, no dependence on age was observed in the quantity of either pigment. For approximately 90% of these, zeaxanthin was dominant. For the remaining 10%, as well as for the seven youngest donors, all below the age of 2, and in prenatal eyes, lutein was the major pigment. In individual retinas, the lutein:zeaxanthin ratio increased from an average of approximately 1:2.4 in the central 0-0.25 mm to over 2:1 in the periphery (8.7-12.2 mm). The variation in this ratio with eccentricity was linearly correlated with the corresponding rod:cone ratio. A selective mechanism of uptake, which results in cones and rods preferentially acquiring zeaxanthin and lutein, respectively, could explain this correlation.