Soft Gel Medium Solidified with Gellan Gum for Preliminary Screening for Root-associating, Free-living Nitrogen-fixing Bacteria Inhabiting the Rhizoplane of Plants
Soft Gel Medium Solidified with Gellan Gum for Preliminary Screening for Root-associating, Free-living Nitrogen-fixing Bacteria Inhabiting the Rhizoplane of Plants
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DOI：
10.1271/bbb.66.2259
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发表时间：
2002-01
期刊：
影响因子：
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通讯作者：
Y. Hashidoko;Motohiko Tada;M. Osaki;S. Tahara
中科院分区：
文献类型：
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作者：
Y. Hashidoko;Motohiko Tada;M. Osaki;S. Tahara
为了初步筛选和鉴定根际微生物群中游离固氮菌，我们使用了Winogradsky's矿物混合物为基础的无氮培养基，培养基中添加0.3%结冷胶。软凝胶培养基使一些参考细菌和野生自由生活的固氮细菌在特定的菌落中生长，包括它们对氧气的反应和它们的运动性变化。因此，结冷胶可能是一种比琼脂糖更好的凝胶基质，用于研究根缔合、自由生活的固氮细菌，以确定它们的特征行为。
For preliminary screening for and characterization of free-living nitrogen-fixing bacteria from rhizoplane microflora, we used Winogradsky's mineral mixture-based nitrogen-free medium solidified with 0.3% gellan gum. The soft gel medium enabled some reference and wild free-living nitrogen-fixing bacteria to grow in characteristic colonies, including their reaction to oxygen and their motility change. Gellan gum is thus likely to be a better gel matrix than agarose for the investigation of root-associating, free-living nitrogen-fixing bacteria to identify their characteristic behaviors.