H6 Influenza Viruses Pose a Potential Threat to Human Health
H6 Influenza Viruses Pose a Potential Threat to Human Health
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DOI：
10.1128/jvi.03292-13
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发表时间：
2014-04-01
影响因子：
5.4
通讯作者：
Chen, Hualan
中科院分区：
文献类型：
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作者：
Wang, Guojun;Deng, Guohua;Chen, Hualan
自从1965年在马萨诸塞州的一只火鸡中首次发现H6亚型流感病毒以来，已从世界各地的野生和家养水生和陆生鸟类中分离出H6亚型流感病毒。1997年以来，中国南部活禽市场频繁检出不同神经氨酸酶(NA)亚型的H6病毒。尽管在过去的几年里已经收集了H6病毒的序列信息，但还没有完全的生物学特征。为了研究H6病毒对人类构成的潜在风险，我们评估了2008年至2011年从中国南部活禽市场分离的一系列H6病毒在哺乳动物中的受体结合偏好、复制和传播性。在测试的257株H6病毒中，有87种病毒识别人型受体。对38株具有代表性的H6病毒的基因组序列分析显示，这些病毒有30种不同的基因型别，表明这些病毒在自然界中正在活跃地传播和重组。在老鼠身上测试的38种病毒中，有37种在肺部有效复制，其中一些会导致轻微疾病；然而，没有一种是致命的。我们还对10种H6病毒在豚鼠体内的直接接触传播进行了检测，发现5种病毒不会传播给接触动物，3种病毒传播给三种接触动物中的一种，2种病毒传播给所有三种接触动物。我们的研究表明，H6禽流感病毒对人类健康构成了明显的威胁，并强调有必要继续监测和评估在自然界中传播的H6流感病毒。近年来，研究和大流行防备主要集中在H5和H7亚型流感病毒上。自1965年在美国首次发现H6亚型流感病毒以来，已从世界各地的野生和家养水生和陆生鸟类中分离出H6亚型流感病毒。自1997年以来，在中国南部的活禽市场频繁检测到H6病毒，但对这些病毒的生物学特性的研究非常有限。在这里，我们评估了从中国南部活禽市场分离的一系列H6病毒的受体结合偏好、复制和在哺乳动物中的传播性，发现34%的病毒能够结合人型受体，其中一些病毒能够有效地传播到动物身上。我们的研究表明，H6病毒对人类健康构成了明显的威胁。
Influenza viruses of the H6 subtype have been isolated from wild and domestic aquatic and terrestrial avian species throughout the world since their first detection in a turkey in Massachusetts in 1965. Since 1997, H6 viruses with different neuraminidase (NA) subtypes have been detected frequently in the live poultry markets of southern China. Although sequence information has been gathered over the last few years, the H6 viruses have not been fully biologically characterized. To investigate the potential risk posed by H6 viruses to humans, here we assessed the receptor-binding preference, replication, and transmissibility in mammals of a series of H6 viruses isolated from live poultry markets in southern China from 2008 to 2011. Among the 257 H6 strains tested, 87 viruses recognized the human type receptor. Genome sequence analysis of 38 representative H6 viruses revealed 30 different genotypes, indicating that these viruses are actively circulating and reassorting in nature. Thirty-seven of 38 viruses tested in mice replicated efficiently in the lungs and some caused mild disease; none, however, were lethal. We also tested the direct contact transmission of 10 H6 viruses in guinea pigs and found that 5 viruses did not transmit to the contact animals, 3 viruses transmitted to one of the three contact animals, and 2 viruses transmitted to all three contact animals. Our study demonstrates that the H6 avian influenza viruses pose a clear threat to human health and emphasizes the need for continued surveillance and evaluation of the H6 influenza viruses circulating in nature.IMPORTANCEAvian influenza viruses continue to present a challenge to human health. Research and pandemic preparedness have largely focused on the H5 and H7 subtype influenza viruses in recent years. Influenza viruses of the H6 subtype have been isolated from wild and domestic aquatic and terrestrial avian species throughout the world since their first detection in the United States in 1965. Since 1997, H6 viruses have been detected frequently in the live poultry markets of southern China; however, the biological characterization of these viruses is very limited. Here, we assessed the receptor-binding preference, replication, and transmissibility in mammals of a series of H6 viruses isolated from live poultry markets in southern China and found that 34% of the viruses are able to bind human type receptors and that some of them are able to transmit efficiently to contact animals. Our study demonstrates that the H6 viruses pose a clear threat to human health.