Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine
Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine
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DOI：
10.1016/j.bbrc.2010.01.126
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发表时间：
2010-03-12
影响因子：
3.1
通讯作者：
Wani, Mohan R.
中科院分区：
文献类型：
--
作者：
Tomar, Geetanjali B.;Srivastava, Rupesh K.;Wani, Mohan R.
骨髓间充质干细胞（Mesenchymal stem cells，MSCs）具有自我更新和分化为多种细胞系的能力。目前，骨髓被认为是间充质干细胞的主要来源：然而，在使用这些间充质干细胞进行细胞治疗时存在一些缺点和限制。在这项研究中，我们证明了人牙龈组织来源的MSC比骨髓来源的MSC有几个优势。牙龈间充质干细胞易于分离、同质化，比骨髓间充质干细胞增殖快，不需要任何生长因子。重要的是，牙龈间充质干细胞显示稳定的形态，并没有松散的MSC特性，在更高的代次。此外，牙龈间充质干细胞在长期培养中保持正常的核型和端粒酶活性，并且不具有致瘤性。因此，我们发现，人牙龈是一个更好的来源，比骨髓间充质干细胞，大量的功能胜任临床级间充质干细胞可以在短时间内产生的细胞治疗再生医学和组织工程。(C)2010年爱思唯尔公司All rights reserved.
Mesenchymal stem cells (MSCs) are capable of self-renewal and differentiation into multiple cell lineages. Presently, bone marrow is considered as a prime source of MSCs: however, there are some drawbacks and limitations in use of these MSCs for cell therapy. In this study, we demonstrate that human gingival tissue-derived MSCs have several advantages over bone marrow-derived MSCs. Gingival MSCs are easy to isolate, homogenous and proliferate faster than bone marrow MSCs without any growth factor. Importantly, gingival MSCs display stable morphology and do not loose MSC characteristic at higher passages. In addition, gingival MSCs maintain normal karyotype and telomerase activity in long-term cultures, and are not tumorigenic. Thus, we reveal that human gingiva is a better source of MSCs than bone marrow, and large number of functionally competent clinical grade MSCs can be generated in short duration for cell therapy in regenerative medicine and tissue engineering. (C) 2010 Elsevier Inc. All rights reserved.