Preparation of chitin nanofibers from squid pen β-chitin by simple mechanical treatment under acid conditions
Preparation of chitin nanofibers from squid pen β-chitin by simple mechanical treatment under acid conditions
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DOI：
10.1021/bm800178b
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发表时间：
2008-07-01
影响因子：
6.2
通讯作者：
Isogai, Akira
中科院分区：
文献类型：
--
作者：
Fan, Yimin;Saito, Tsuguyuki;Isogai, Akira
开发了一种制备截面宽度为3 ~ 4 nm、长度至少为几微米的个体化甲壳素纳米纤维的方法。制备高长径比甲壳素纳米纤维的关键因素有：(1)以鱿鱼笔β -甲壳素为原料；(2)将β -甲壳素在pH为3-4、浓度为0.1-0.3%的水中超声处理几分钟。该方法可获得鱿鱼笔-几丁质纳米纤维在水中透明、高粘性的分散体。在纳米纤维转化过程中，甲壳素分子上没有发生n -去乙酰化。此外，由于纳米纤维的转化，β -几丁质的结晶度指数从0.51下降到0.37，但仍保持了原有的晶体结构。鱿鱼笔-几丁质晶体表面的C2氨基在酸性条件下阳离子化是制备纳米纤维的必要条件。
A procedure for preparing individualized chitin nanofibers 3-4 nm in cross-sectional width and at least a few microns in length was developed. The key factors to prepare the chitin nanofibers with such high aspect ratios are as follows: (1) squid pen beta-chitin is used as the starting material and (2) ultrasonication of the beta-chitin in water at pH 3-4 and 0.1-0.3% consistency for a few minutes. Transparent and highly viscous dispersions of squid pen beta-chitin nanofibers in water can be obtained by this method. No N-deacetylation occurs on the chitin molecules during the nanofiber conversion procedure. Moreover, the original crystal structure of beta-chitin is maintained, although crystallinity index decreases from 0.51 to 0.37 as a result of the nanofiber conversion. Cationization of the C2 amino groups present on the crystallite surfaces of the squid pen beta-chitin under acid conditions is necessary for preparing the nanofibers.