Prevalence and metronidazole resistance of Trichomonas vaginalis among Japanese women in 2021
Prevalence and metronidazole resistance of Trichomonas vaginalis among Japanese women in 2021
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DOI：
10.1016/j.ijregi.2023.02.007
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发表时间：
2023-06-01
期刊：
影响因子：
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通讯作者：
Hisaeda, Hajime
中科院分区：
文献类型：
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作者：
Saito-Nakano, Yumiko;Umeki, Yuko;Hisaeda, Hajime
目的：阴道毛滴虫是世界范围内最常见的性传播寄生虫。然而，没有监测系统存在监测T。在日本的迷走神经病例和耐药性。方法：收集有或无疑似弓形虫症状妇女的宫颈细胞学阴道拭子。这些拭子用于检测T.使用特异性聚合酶链反应检测人乳头瘤病毒（HPV）和白色念珠菌。临床分离的T.采用文献报道的最小致死浓度（MLC）和新建立的半数最大抑制浓度（IC 50）值对100例迷走神经痛患者进行甲硝唑敏感性试验。研究人群中的迷走神经损伤为4.2%（5/119; 95%置信区间[Cl]，1.5-9.7）。无症状感染者占全部病例的60%（3/5）。迷走神经感染所有T。阳性者合并HPV感染，而不合并C。白色念珠菌。5个临床T.迷走神经炎分离株对甲硝唑敏感，采用MLC值进行评价。定量IC 50值显示，这些临床分离株中的两个表现出甲硝唑敏感性降低。结论：本研究首次证实了T。日本女人的流浪汉甲硝唑对T.迷走神经检查能够精确定量评价甲硝唑敏感T.流浪汉
Objectives: Trichomonas vaginalis is the most prevalent sexually transmitted parasite worldwide. However, no surveillance system exists to monitor T. vaginalis cases and drug resistance in Japan. Methods: Cervical cytology vaginal swabs were collected from women with and without suspected symptoms of T. vaginalis infection; these swabs were used for the detection of T. vaginalis , human papillomavirus (HPV), and Candida albicans using specific polymerase chain reaction. Clinical isolates of T. vaginalis were subjected to metronidazole susceptibility tests using the previously reported minimal lethal concentration (MLC) and newly established half-maximal inhibitory concentration (IC 50 ) values.Results: The prevalence of T. vaginalis in the study population was 4.2% (5/119; 95% confidence interval [Cl], 1.5-9.7). Additionally, asymptomatic infection constituted 60% (3/5) of all cases of T. vaginalis infection. All T. vaginalis -positive patients were coinfected with HPV but not C. albicans . Five clinical T. vaginalis isolates showed metronidazole susceptibility, which was evaluated using MLC values. The quantitative IC 50 values revealed that two of these clinical isolates exhibited a decreased metronidazole susceptibility. Conclusion: This is the first study to demonstrate the prevalence of T. vaginalis in Japanese women. The IC 50 values of metronidazole against T. vaginalis enabled the precise and quantitative evaluation of metronidazole-susceptible T. vaginalis .