IL-17 enhances the susceptibility of U-2OS osteosarcoma cells to NK cell lysis
IL-17 enhances the susceptibility of U-2OS osteosarcoma cells to NK cell lysis
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DOI：
10.1046/j.1365-2249.2003.02234.x
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发表时间：
2003-09-01
影响因子：
4.6
通讯作者：
Facchini, A
中科院分区：
文献类型：
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作者：
Honorati, MC;Neri, S;Facchini, A
我们研究了促炎细胞因子白细胞介素17 （IL-17）对人NK细胞溶解骨肉瘤细胞的影响。NK细胞和U-2 OS、MG-63、HOS骨肉瘤细胞系表达IL-17受体，其中U-2 OS表达量最高。NK细胞与IL-17预孵育对骨肉瘤的细胞毒性没有影响，但与IL-17预孵育后，U-2 OS对骨肉瘤的细胞毒性增加。在IL-17处理的U-2 OS骨肉瘤细胞中，FACS分析显示，在所检测的粘附分子组中，纤维连接蛋白的表达增加，抗纤维连接蛋白抗体处理降低了NK细胞毒性。干扰素γ (ifn - γ)处理和ifn - γ /IL-17处理的U-2 OS之间的比较显示，ifn - γ /IL-17处理的U-2 OS对NK裂解的敏感性降低，与CD49f表达减少有关。IL-17似乎是U-2 OS细胞上NK粘附分子的调节剂，但在NK裂解时与ifn - γ拮抗。
We investigated the effect of the proinflammatory cytokine interleukin 17 (IL-17) on the lysis of osteosarcoma cells by human NK cells. NK cells and U-2 OS, MG-63, HOS osteosarcoma cell lines express the IL-17 receptor, the highest amount being found on U-2 OS. Pre-incubation of NK cells with IL-17 did not affect the cytotoxicity against osteosarcomas, that was increased when U-2 OS were preincubated with IL-17. In IL-17 treated U-2 OS osteosarcoma cells FACS analysis demonstrated an increased expression of fibronectin among the panel of adhesion molecules assayed, and the treatment with anti-fibronectin antibodies decreased the NK cytotoxicity. The comparison between interferon gamma (IFN-gamma) treated and IFN-gamma/IL-17-treated U-2 OS showed a decreased susceptibility to NK lysis associated with a reduced expression of CD49f on U-2 OS treated with IFN-gamma/IL-17. IL-17 appears to be a modulator of NK adhesion molecules on U-2 OS cells but antagonizes with IFN-gamma on NK lysis.