Fatty acid binding protein 4 is expressed in distinct endothelial and non-endothelial cell populations in glioblastoma
Fatty acid binding protein 4 is expressed in distinct endothelial and non-endothelial cell populations in glioblastoma
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DOI：
10.1111/j.1365-2990.2011.01237.x
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发表时间：
2012-08-01
影响因子：
5
通讯作者：
Cataltepe, S.
中科院分区：
文献类型：
--
作者：
Cataltepe, O.;Arikan, M. C.;Cataltepe, S.
O. Cataltepe， M. C. Arikan， E. Ghelfi， C. Karaaslan， Y. Ozsurekci， K. Dresser， Y. Li， T. W. Smith和S. Cataltepe（2012）胶质母细胞瘤中不同内皮细胞和非内皮细胞群中脂肪酸结合蛋白4的表达目的：胶质母细胞瘤（GBM）是成人中最常见和侵袭性的原发性脑肿瘤。血管新生和血管新生在GBMs的进展中起关键作用。脂肪酸结合蛋白4 （FABP4）是游离脂肪酸的细胞内伴侣。在几种正常组织的微血管内皮细胞（ECs）中检测到FABP4，并促进ECs的增殖。本研究的目的是表征FABP4在GBMs中的组织分布模式。方法：对肿瘤石蜡包埋切片进行FABP4免疫组化，分析FABP4免疫反应的强度和分布。双免疫荧光法对fabp4阳性细胞进行详细表征。结果：正常脑组织切片无FABP4免疫反应性。在I级、II级、III级和IV级胶质肿瘤中，分别有33%、43%、64%和89%检测到fabp4阳性细胞。因此，fabp4阳性细胞在GBMs中的比例明显高于低级别胶质瘤。一般来说，表达fabp4的细胞呈非均匀分布，在胶质肿瘤中作为热点。在一部分血管内皮细胞和一些非内皮细胞中检测到FABP4的表达。结论：FABP4在GBMs中的表达比例明显高于正常脑组织和低级别胶质肿瘤。FABP4在一些肿瘤内皮细胞中表达，也在胶质肿瘤的非内皮细胞中表达。由于FABP4促进了内皮细胞的增殖，在GBM-ECs中检测到FABP4，而在正常脑ECs中检测不到，这表明FABP4可能在GBMs相关的强大血管生成中发挥作用。
O. Cataltepe, M. C. Arikan, E. Ghelfi, C. Karaaslan, Y. Ozsurekci, K. Dresser, Y. Li, T. W. Smith and S. Cataltepe (2012) Neuropathology and Applied Neurobiology38, 400410 Fatty acid binding protein 4 is expressed in distinct endothelial and non-endothelial cell populations in glioblastoma Aims: Glioblastoma (GBM) is the most common and aggressive primary brain tumour in adults. Angiogenesis and vasculogenesis play key roles in progression of GBMs. Fatty acid binding protein 4 (FABP4) is an intracellular chaperone for free fatty acids. FABP4 is detected in microvascular endothelial cells (ECs) in several normal tissues and promotes proliferation of ECs. The goal of this study was to characterize the tissue distribution pattern of FABP4 in GBMs. Methods: Immunohistochemistry for FABP4 was performed on paraffin-embedded tumour sections and the intensity and distribution of FABP4 immunoreactivity were analysed. Double immunofluorescence was employed for detailed characterization of FABP4-positive cells. Results: FABP4 immunoreactivity was absent in normal brain tissue sections. FABP4-positive cells were detected in 33%, 43%, 64% and 89% of Grade I, Grade II, Grade III and Grade IV glial tumours, respectively. Thus, the percentage of FABP4-positive cells in GBMs was significantly higher than lower-grade gliomas. In general, FABP4-expressing cells were distributed in a non-homogenous pattern, as hot spots in glial tumours. FABP4 expression was detected in a subset of vascular ECs as well as some non-ECs. Conclusion: FABP4 is expressed in a significantly higher percentage of GBMs in comparison to both normal brain tissues and lower-grade glial tumours. FABP4 is expressed in some tumour ECs as well as non-ECs in glial tumours. As FABP4 promotes proliferation of ECs, detection of FABP4 in GBM-ECs, but not normal brain ECs suggests that FABP4 may play a role in the robust angiogenesis associated with GBMs.