Cells from subcutaneous tissues contribute to scarless skin regeneration in Xenopus laevis froglets
Cells from subcutaneous tissues contribute to scarless skin regeneration in Xenopus laevis froglets
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来自皮下组织的细胞有助于非洲爪蟾幼蛙的无疤痕皮肤再生
DOI：
10.1002/dvdy.24520
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发表时间：
2017
影响因子：
2.5
通讯作者：
Yokoyama Hitoshi
中科院分区：
文献类型：
--
作者：
Otsuka-Yamaguchi Rina;Kawasumi-Kita Aiko;Kudo Nanako;Izutsu Yumi;Tamura Koji;Yokoyama Hitoshi
背景：哺乳动物受伤后无法再生真皮和其他皮肤结构，而是会形成疤痕。然而，非洲爪蟾在皮肤切除后可以在四肢和躯干上再生无疤痕皮肤，包括真皮和分泌腺。四肢和躯干的皮下组织主要由肌肉组成。尽管皮肤损伤下的皮下组织看起来杂乱无章，但这些皮下组织对皮肤再生的细胞贡献仍不清楚。结果：我们将近交系 J 品系与绿色荧光蛋白 (GFP) 标记的转基因爪蟾杂交，获得了具有 GFP 阴性皮肤和 GFP 标记皮下组织的嵌合蛙，并且在变态后不受免疫排斥的影响。我们发现来自皮下组织的 GFP 阳性细胞有助于切除损伤后皮肤的再生，尤其是真皮。我们还表明，头部的皮肤（位于骨骼而不是肌肉之上）也可以完全再生皮肤结构。结论：源自皮下组织的细胞（至少在躯干区域）有助于皮肤再生，并且可能对于皮肤再生至关重要。对两栖动物中有助于皮肤再生的皮下组织来源的细胞进行表征，可能会诱导出能够在哺乳动物中再生完整皮肤结构而不留下疤痕的细胞。Developmental Dynamics 246:585–597, 2017。© 2017 Wiley periodicals, Inc.
Background: Mammals cannot regenerate the dermis and other skin structures after an injury and instead form a scar. However, aXenopus laevisfroglet can regenerate scarless skin, including the dermis and secretion glands, on the limbs and trunk after skin excision. Subcutaneous tissues in the limbs and trunk consist mostly of muscles. Although subcutaneous tissues beneath a skin injury appear disorganized, the cellular contribution of these underlying tissues to skin regeneration remains unclear.Results: We crossed the inbred J strain with a green fluorescent protein (GFP)‐labeled transgenicXenopusline to obtain chimeric froglets that have GFP‐negative skin and GFP‐labeled subcutaneous tissues and are not affected by immune rejection after metamorphosis. We found that GFP‐positive cells from subcutaneous tissues contributed to regenerating the skin, especially the dermis, after an excision injury. We also showed that the skin on the head, which is over bone rather than muscle, can also completely regenerate skin structures.Conclusions: Cells derived from subcutaneous tissues, at least in the trunk region, contribute to and may be essential for skin regeneration. Characterizing the subcutaneous tissue–derived cells that contribute to skin regeneration in amphibians may lead to the induction of cells that can regenerate complete skin structures without scarring in mammals.Developmental Dynamics 246:585–597, 2017. © 2017 Wiley Periodicals, Inc.