Venomic study on cone snails (Conus spp.) from South Africa
Venomic study on cone snails (Conus spp.) from South Africa
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DOI：
10.1016/j.toxicon.2010.09.009
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发表时间：
2011-01-01
期刊：
影响因子：
2.8
通讯作者：
Mebs, Dietrich
中科院分区：
文献类型：
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作者：
Kauferstein, Silke;Porth, Christine;Mebs, Dietrich
采用MALDI-TOF质谱法对采集自南非东海岸的6种圆锥虫（Conus coronatus、Conus lividus、Conus mozbicus f. lautus、Conus pictus、Conus sazanka、Conus tinanus）的毒液进行了分析。鉴定出的分子质量在56 ~ 151之间，大多数在1000 ~ 2500da之间。在6种毒液中，冠状冠蛾和山棘冠蛾的肽团相似度在0 ~ 27%之间。在对菲律宾收集的6种圆锥虫的毒液进行的一项研究中，相同团块的百分比仅在零到9%之间。除冠状锥体毒仅能阻断α 4 β 2和α 7 nachr外，南非锥状锥体毒均能拮抗大鼠神经元烟碱乙酰胆碱受体α 3 β 2、α 4 β 2和α 7 nachr。高效液相色谱法分离出了一种对三种受体亚型都有活性的肽。它由16个氨基酸残基组成，通过两个二硫桥交联，通过串联质谱从头测序发现：GGCCSHPACQNNPDYC。翻译后修饰包括c端酰胺化和酪氨酸磺化。新肽是α -螺毒素家族的成员，是nachr的竞争性拮抗剂。对众多圆锥圆锥属物种的16S RNA进行系统发育分析，明确了南非特有圆锥圆锥属物种的进化位置，为其密切的亲缘关系提供了第一个证据。(C) 2010 Elsevier Ltd.版权所有。
From six Conus species (Conus coronatus, Conus lividus, Conus mozambicus f. lautus, Conus pictus, Conus sazanka, Con us tinianus) collected off the eastern coast of South Africa the venoms were analyzed using MALDI-TOF mass spectrometry. Between 56 and 151 molecular masses most in a range of 1000 to 2500 Da, were identified. Among the six venoms, between 0 and 27% (C coronatus versus C. sazanka) of the peptide masses were found to be similar. In a study on venoms from 6 Conus species collected in the Philippines, the percentage of identical masses was between none and 9% only. The venoms from the South African Conus species antagonized the rat neuronal nicotinic acetylcholine receptors (nAChRs) alpha 3 beta 2, alpha 4 beta 2, and alpha 7, except for C coronatus venom that blocked the alpha 4 beta 2 and alpha 7 nAChRs only. HPLC-fractionation of C tinianus venom led to the isolation of a peptide that is active on all three receptor subtypes. It consists of 16 amino acid residues crosslinked by two disulfide bridges as revealed by de novo sequencing using tandem mass spectrometry: GGCCSHPACQNNPDYC. Posttranslational modifications include C-terminal amidation and tyrosine sulfation. The new peptide is a member of the alpha-conotoxin family that are competitive antagonists of nAChRs. Phylogenetic analysis of the 16S RNA from numerous Conus species has clarified the evolutionary position of endemic South African Conus species and provided the first evidence for their close genetic relationships. (C) 2010 Elsevier Ltd. All rights reserved.