Evaluation of rat ultrasonic vocalizations as predictors of the conditioned aversive effects of drugs
Evaluation of rat ultrasonic vocalizations as predictors of the conditioned aversive effects of drugs
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DOI：
10.1007/s002130100685
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发表时间：
2001-04-01
影响因子：
3.4
通讯作者：
Shippenberg, TS
中科院分区：
文献类型：
--
作者：
Burgdorf, J;Knutson, B;Shippenberg, TS
理论基础：由于预测厌恶结果的线索可以在成年大鼠中引起回避和20khz超声发声（usv），因此20khz超声发声也可以指示药物的条件厌恶效应。目的：我们评估暴露于与药物相关的室是否会选择性地增加大鼠20 kHz usv而不是50 kHz usv。方法：给大鼠注射纳洛酮（NAL）或氯化锂（LiCl）后置于一个隔间，或与生理盐水（VEH）后置于另一个隔间，进行3次50 min的调理。在随后的15分钟测试期间，记录20 kHz usv， 50 kHz usv和在每个腔室中花费的时间，在此期间，大鼠可以进入两个腔室（专家1）或被限制在药物或veh配对的腔室（专家2）。结果：在实验1中，NAL（0.3和3.0 mg/kg）和LiCl（10和30 mg/kg）处理的动物增加了20.1；与veh条件对照相比，药物配对室的usv为Hz。在实验2中，与veh条件对照相比，高剂量的NAL和LiCl也增加了20 kHz usv的发射，减少了50 kHz usv的发射。结论：在大鼠中，与具有厌恶效应的药物相关的线索增加了20 kHz usv，减少了50 kHz usv，表明usv可能为预测药物的条件厌恶效应提供了一个有用的模型。
Rationale: Since cues that predict aversive outcomes can elicit both avoidance and 20 kHz ultrasonic vocalizations (USVs) in adult rats, 20 kHz USVs may also index the conditioned aversive effects of drugs. Objective: We evaluated whether exposure to compartments associated with drugs With aversive effects would selectively increase 20 but not 50 kHz USVs in rats. Method: Rats were injected with naloxone (NAL) or lithium chloride (LiCl) and placed in one compartment or with Saline (VEH) and placed in another compartment for three 50-min conditioning sessions. 20 kHz USVs, 50 kHz USVs, and time spent in each chamber were recorded during subsequent 15-min testing sessions during which rats had access to both compartments (expt 1) or were confined to the drug- or VEH-paired compartment (expt 2). Results: In expt 1, animals conditioned either with NAL (0.3 and 3.0 mg/kg) or LiCl (10 and 30 mg/kg) emitted increased 20 1;Hz USVs in the drug-paired compartment, relative to VEH-conditioned controls. Conditioning with high doses of both drugs also increased conditioned place aversion and decreased emission of 50 kHz USVs, In expt 2, restriction of animals to the compartment paired with high doses of NAL and LiCl also increased emission of 20 kHz USVs and decreased 50 kHz USVs, relative to VEH-conditioned controls. Conclusions: In rats, cues associated with drugs with aversive effects increase 20 kHz USVs and decrease 50 kHz USVs, suggesting that USVs may provide a useful model for predicting the conditioned aversive effects of drugs.