Structure and developmental expression of hatching enzyme genes of the Japanese eel Anguilla japonica:: an aspect of the evolution of fish hatching enzyme gene
Structure and developmental expression of hatching enzyme genes of the Japanese eel Anguilla japonica:: an aspect of the evolution of fish hatching enzyme gene
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DOI：
10.1007/s00427-004-0397-1
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发表时间：
2004-04-01
影响因子：
2.4
通讯作者：
Yasumasu, S
中科院分区：
文献类型：
--
作者：
Hiroi, J;Maruyama, K;Yasumasu, S
我们从日本鳗胚胎中分离到7个克隆。每个推导的氨基酸序列由一个信号肽、一个前肽和一个属于ASTIN蛋白酶家族的成熟酶部分组成。系统发育分析表明，这些鳗鱼的酶与青竹的高绒毛膜分解酶(HCE)相似，与斑马鱼Danio rerio和Masu鲑鱼Oncorhynchus Masou的孵化酶相似。这些硬骨鱼的孵化酶属于青竹类，而不是青竹的另一种孵化酶-青竹低绒毛膜溶血酶(LCE)。Southern杂交分析表明，鳗鱼孵化酶基因与青杨HCE基因类似，为多拷贝基因。然而，其中一个鳗鱼孵化酶基因由8个外显子和7个内含子组成，外显子-内含子的组织结构类似于青蛙LCE基因，是一个单拷贝基因。对鱼类孵化酶基因的分子进化进行了讨论。此外，整体原位杂交和免疫细胞化学显示，鳗鱼孵化酶首先在早期神经胚前脑前枕部表达，最后在后期胚胎卵黄囊的细胞团中表达。鳗鱼孵化腺细胞的早期分化特征与青鱼、三文鱼和斑马鱼相似，但不同鱼类的腺细胞的最终位置不同。
We isolated seven cDNA clones from embryos of the Japanese eel Anguilla japonica. Each deduced amino acid sequence consisted of a signal peptide, a propeptide and a mature enzyme portion belonging to the astacin protease family. A phylogenetic analysis showed that the eel enzymes resembled the high choriolytic enzyme (HCE) of medaka Oryzias latipes, and the hatching enzymes of the zebra fish Danio rerio and masu salmon Oncorhynchus masou. Hatching enzymes of these teleosts belonged to the group of the medaka HCE, and not the medaka low choriolytic enzyme (LCE), another hatching enzyme of medaka. Southern blot analysis showed that the genes of the eel hatching enzymes were multicopy genes like the medaka HCE genes. However, one of the eel hatching enzyme genes comprised eight exons and seven introns, and the exon-intron organization was similar to the medaka LCE gene, which is a single-copy gene. The molecular evolution of the fish hatching enzyme genes is discussed. In addition, whole-mount in situ hybridization and immunocytochemistry showed that the eel hatching enzyme was first expressed in the pillow anterior to the forebrain of early neurula, and finally in the cell mass on the yolk sac of later stage embryos. The early differentiation profile of eel hatching gland cells was similar to that of medaka, masu salmon and zebrafish, whereas the final location of the gland cells was different among fishes.