Nucleotide sequence of the control regions for the glnA and glnL genes of Salmonella typhimurium
Nucleotide sequence of the control regions for the glnA and glnL genes of Salmonella typhimurium
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鼠伤寒沙门氏菌glnA和glnL基因控制区的核苷酸序列
DOI：
10.1128/jb.155.1.82-89.1983
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发表时间：
1983
影响因子：
3.2
通讯作者：
J. Brenchley
中科院分区：
文献类型：
--
作者：
R. Hanau;K. Raju;Kodurij;H. Nancy;J. Brenchley
我们已经部分表征了编码谷氨酰胺合成酶的DNA片段在鼠伤寒沙门氏菌。限制性酶切图谱和RNA聚合酶结合研究确定了片段内的两个区域，当与pMC1403中的lacZ融合时，其表现出启动子活性，pMC1403是一种用于检测转录和翻译控制信号的质粒。DNA序列分析显示，一个区域编码对应于谷氨酰胺合成酶蛋白的氨基末端的氨基酸。第二个区域编码对应于谷氨酰胺合成酶的羧基末端的氨基酸，随后是含有理想的Pribnow七聚体和可能的翻译起始信号的330个核苷酸的序列。该区域的位置类似于大肠杆菌中鉴定的glnL基因的起始位置，并且很可能Pribnow七聚体是glnL基因的RNA聚合酶结合位点。
We have partially characterized a DNA fragment encoding glutamine synthetase in Salmonella typhimurium. Restriction mapping and RNA polymerase binding studies identified two regions within the fragment which exhibit promoter activity when fused to lacZ in pMC1403, a plasmid used to detect transcriptional and translational control signals. DNA sequence analysis revealed that one region encodes amino acids corresponding to the amino terminus of the glutamine synthetase protein. The second region codes for the amino acids corresponding to the carboxy terminus of glutamine synthetase followed by a 330-nucleotide sequence containing an ideal Pribnow heptamer and a possible translation initiation signal. The location of this region is analogous to the position of the beginning of the glnL gene identified in Escherichia coli, and it is likely that the Pribnow heptamer is the RNA polymerase binding site for the glnL gene.