Neutralization of interleukin-11 activity decreases osteoclast formation and increases cancellous bone volume in ovariectomized mice.
Neutralization of interleukin-11 activity decreases osteoclast formation and increases cancellous bone volume in ovariectomized mice.
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中和白细胞介素 11 活性可减少去势小鼠的破骨细胞形成并增加松质骨体积。
作者：
S. Shaughnessy;Kimberly J. Walton;Paula A. Deschamps;Martin K Butcher;S. Beaudin
白细胞介素-11（IL-11）是否有助于雌激素缺乏状态下的骨丢失的问题尚未确定。因此，我们将卵巢切除（OVX）小鼠随机分组，每天一次腹膜内注射绵羊抗鼠IL-11 Ab或正常绵羊IgG（NSIG），持续21天，然后使用骨组织形态计量学确定对骨的影响。在这里，我们报告说，治疗OVX小鼠与抗IL-11抗体显着增加骨小梁宽度和松质骨体积。通过骨小梁表面被类骨质覆盖的百分比和骨形成速率测量的成骨细胞活性在用抗IL-11 Ab处理后也显著增加。相反，用抗IL-11 Ab治疗OVX小鼠显著降低破骨细胞数量和活性。破骨细胞形成和活性的体外测定证实了组织形态学数据。因此，从抗IL-11 Ab处理的OVX小鼠中分离的骨髓细胞在培养物中形成的破骨细胞和骨吸收比从未处理或NSIG处理的OVX小鼠中分离的骨髓细胞少。基于这些结果，我们得出结论，IL-11有助于在雌激素缺乏状态下观察到的骨丢失。
The issue of whether interleukin-11 (IL-11) contributes to bone loss during states of estrogen deficiency has not been previously determined. We therefore randomized ovariectomized (OVX) mice to once daily interperitoneal injections of either sheep anti-murine IL-11 Ab or normal sheep IgG (NSIgG) for 21 days, and then determined the effects on bone using bone histomorphometry. Here we report that treatment of OVX mice with anti-IL-11 Ab significantly increases both trabecular width and cancellous bone volume. Osteoblast activity, as measured by the percentage of trabecular surface covered by osteoid and rates of bone formation, were also significantly increased following treatment with anti-IL-11 Ab. In contrast, treatment of OVX mice with anti-IL-11 Ab significantly decreased both osteoclast number and activity. Ex-vivo assays of osteoclast formation and activity confirmed the histomorphometric data. Thus, bone marrow cells isolated from anti-IL-11 Ab treated OVX mice formed fewer osteoclasts and resorbed less bone in culture than did marrow cells isolated from either untreated or NSIgG-treated OVX mice. Based on these results we conclude that IL-11 contributes to the bone loss which is observed during states of estrogen deficiency.
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Interleukin-11 调节与 interleukin-6 相同的血浆蛋白基因的肝脏表达。
DOI：
--
发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Baumann,H;Schendel,P
通讯作者：
Schendel,P
影响因子：
15.9
作者：
GIRASOLE, G;PASSERI, G;MANOLAGAS, SC
通讯作者：
MANOLAGAS, SC
DOI：
10.1073/pnas.87.19.7512
发表时间：
1990-10-01
影响因子：
11.1
作者：
PAUL, SR;BENNETT, F;YANG, YC
通讯作者：
YANG, YC
影响因子：
15.9
作者：
Bellido, T;Stahl, N;Manolagas, SC
通讯作者：
Manolagas, SC