RF heating of magnetic nanoparticles improves the thawing of cryopreserved biomaterials
RF heating of magnetic nanoparticles improves the thawing of cryopreserved biomaterials
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DOI：
10.1142/s2339547814500204
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发表时间：
2014-09-01
期刊：
影响因子：
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通讯作者：
Bischof, John C.
中科院分区：
文献类型：
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作者：
Etheridge, Michael L.;Xu, Yi;Bischof, John C.
虽然玻璃化冷冻保存具有很大的前景，但由于扩散性热和传质限制，实际应用仅限于较小的系统（细胞和薄组织），这通常表现为解冻期间的失透和破裂故障。在这里，然后，我们描述了一种新的方法，用于快速和均匀地加热冷冻保存的生物标本与射频（RF）激发的磁性纳米粒子（mNPs）。重要的是，加热速率可以比传统的边界加热技术提高数倍，并且与样本大小无关。初始差示扫描量热法研究表明，mNP的添加对冷冻保护剂系统本身的冻融行为的影响最小。然后，在水溶液和冷冻保护剂溶液中的原理验证实验证明了以足够高的速率加热以减轻或消除失活的能力（数百摄氏度/分钟），并且使用缩放的传热模型来说明这种创新方法的潜力。最后，X射线微计算机断层扫描（micro-CT）作为一种计划和质量控制工具进行了研究，其中基于密度的测量能够量化冷冻保护剂浓度、mNP浓度和冷冻状态（即结晶与玻璃化）的变化。
While vitrified cryopreservation holds great promise, practical application has been limited to smaller systems (cells and thin tissues) due to diffusive heat and mass transfer limitations, which are typically manifested as devitrification and cracking failures during thaw. Here then we describe a new approach for rapidly and uniformly heating cryopreserved biospecimens with radiofrequency (RF) excited magnetic nanoparticles (mNPs). Importantly, heating rates can be increased several fold over conventional boundary heating techniques and are independent of sample size. Initial differential scanning calorimetry studies indicate that the addition of the mNPs has minimal impact on the freeze-thaw behavior of the cryoprotectant systems themselves. Then proof-of-principle experiments in aqueous and cryoprotectant solutions demonstrate the ability to heat at rates high enough to mitigate or eliminate devitrifi cation (hundreds of degrees C/min) and scaled heat transfer modeling is used to illustrate the potential of this innovative approach. Finally, X-ray micro- computed-tomography (micro-CT) is investigated as a planning and quality control tool, where the density-based measurements are able to quantify changes in cryoprotectant concentration, mNP concentration, and the frozen state (i.e. crystallized versus vitrified).