Characterisation of humoral immune responses in dogs vaccinated with irradiated Ancylostoma caninum
Characterisation of humoral immune responses in dogs vaccinated with irradiated Ancylostoma caninum
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DOI：
10.1016/s0165-2427(03)00006-0
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发表时间：
2003-03-20
影响因子：
1.8
通讯作者：
Sexton, JL
中科院分区：
文献类型：
--
作者：
Boag, PR;Parsons, JC;Sexton, JL
Infection with Ancylostoma caninum, an intestinal hookworm of dogs, can cause debilitating and potentially life-threatening disease. In the current study, protective immunity to hookworm infection was induced in dogs following vaccination with irradiation-attenuated third-stage larvae (L3) with significant reductions in both worm (P < 0.03) and faecal egg counts (P < 0.0004) following a challenge infection. Vaccination with irradiated L3 and challenge with infective L3 stimulated a dominant antibody response to antigens of less than 20 kDa in an excretory/secretory extract from adult parasites. Immuno-screening of an adult A. caninum cDNA library with antisera from the vaccine trial identified a number of clones. The three clones with the strongest immunoreactivity proved to be identical and encoded a peptide with similarity to the N-terminal domain of the tissue matrix metalloproteinase inhibitor (TIMP)-2 mammalian tissue metalloproteinase inhibitor family. (C) 2003 Elsevier Science B.V. All rights reserved.
