KIM185, A MONOCLONAL-ANTIBODY TO CD18 WHICH INDUCES A CHANGE IN THE CONFORMATION OF CD18 AND PROMOTES BOTH LFA-1-DEPENDENT AND CR3-DEPENDENT ADHESION
KIM185, A MONOCLONAL-ANTIBODY TO CD18 WHICH INDUCES A CHANGE IN THE CONFORMATION OF CD18 AND PROMOTES BOTH LFA-1-DEPENDENT AND CR3-DEPENDENT ADHESION
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DOI：
10.1002/eji.1830230925
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发表时间：
1993-09-01
影响因子：
5.4
通讯作者：
ROBINSON, M
中科院分区：
文献类型：
--
作者：
ANDREW, D;SHOCK, A;ROBINSON, M
未活化的外周血白细胞几乎没有与其他细胞或基质成分结合的倾向，而在炎症介质存在的情况下，粘附相互作用会迅速增加。粘附分子的 Leu-CAM（β2 整合素）家族已被证明可以介导各种诱导的粘附事件。在这里，我们描述了一种针对 CD18 的单克隆抗体 KIM185，它通过 CD11a 途径刺激 JY 细胞同型聚集，并通过 CD11b 依赖性机制诱导中性粒细胞粘附到蛋白质包被的塑料上。该抗体识别与之前描述的 KIM127 抗体不同的表位，并且有证据表明 KIM185 的结合可以引起 CD18 分子构象的变化。
Unactivated peripheral blood leukocytes show little tendency to bind to other cells or matrix components, whilst, in the presence of inflammatory mediators, adhesive interactions can rapidly increase. The Leu-CAM (beta2 integrin) family of adhesion molecules have been shown to mediate a variety of these induced adhesion events. Here we describe a monoclonal antibody against CD18, KIM185, which stimulates JY cell homotypic aggregation by a CD11a pathway as well as inducing the adherence ot neutrophils to protein-coated plastic by a CD11b-dependent mechanism. The antibody recognizes an epitope distinct from the previously described KIM127 antibody and evidence is presented that the binding of KIM185 can cause a change in the conformation of the CD18 molecule.