DIFFERENCES IN TRANSFERRIN RESPONSE AND NUMBERS OF TRANSFERRIN RECEPTORS IN RAT AND HUMAN MAMMARY-CARCINOMA LINES OF DIFFERENT METASTATIC POTENTIALS
DIFFERENCES IN TRANSFERRIN RESPONSE AND NUMBERS OF TRANSFERRIN RECEPTORS IN RAT AND HUMAN MAMMARY-CARCINOMA LINES OF DIFFERENT METASTATIC POTENTIALS
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DOI：
10.1002/jcp.1041560128
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发表时间：
1993-07-01
影响因子：
5.6
通讯作者：
NICOLSON, GL
中科院分区：
文献类型：
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作者：
INOUE, T;CAVANAUGH, PG;NICOLSON, GL
我们以前发现，转铁蛋白（Tf）差异刺激小鼠黑色素瘤的高转移性变异株的生长，这些高转移性细胞的细胞表面也有更多的Tf受体。在本研究中，我们发现高转移性大鼠乳腺癌细胞系在增殖试验中对Tf的反应也有差异，并且细胞单层结合Tf与其转移潜力有关（MTPaB 10> MTPaB 5> MTLn 3> MTLn 2> MTC > MTF 7> MTPa）。脑定殖系PaB 10和PaB 5对Tf的反应性最强，并且具有最高数量的Tf受体。不同的人乳腺癌细胞系在增殖试验中也对Tf有不同的反应，并将不同数量的Tf结合到其细胞表面Tf受体上。转铁蛋白结合，而不是生长反应，与选自人MCF-7系列的细胞系的转移性和侵袭性相关（MCF 7/LCC 2> MCF 7/LCC 1> MCF 7）。在检查转铁蛋白结合和来自人MDA系列的细胞系的生长反应时，Tf结合和生长反应为MDA 231> MDA 435> MDA 468。在裸鼠测定中，细胞系MDA 435和MDA 231是转移性的，而细胞系MDA 468不是。Scatchard分析表明，在大鼠和人乳腺细胞系上存在单一类别的Tf受体。结果表明，肿瘤细胞显示不同的转移特性，可能会表达不同数量的Tf受体和不同的响应生长因子，如Tf。(C)1993 Wiley-Liss，Inc.
We previously found that transferrin (Tf) differentially stimulated the growth of highly metastatic variant lines of murine melanoma and that these highly metastatic cells also had greater numbers of Tf receptors on their cell surfaces. In the present study we found that highly metastatic rat mammary adenocarcinoma cell lines also responded differentially to Tf in proliferation assays, and cell monolayers bound Tf in relation to their metastatic potential (MTPaB10 > MTPaB5 > MTLn3 > MTLn2 > MTC > MTF7 > MTPa). The brain-colonizing lines PaB10 and PaB5 were the most responsive to Tf and had the highest numbers of Tf receptors. Different human breast cancer cell lines also responded differentially to Tf in proliferation assays and bound different amounts of Tf to their cell surface Tf receptors. Transferrin binding, but not growth response, correlated with metastatic and invasive properties of lines selected from the human MCF-7 series (MCF7/LCC2 > MCF7/LCC1 > MCF7). In examining the transferrin binding and growth response of lines from the human MDA series, the Tf binding and growth response was MDA231 > MDA435 > MDA468. The lines MDA435 and MDA231 were metastatic in nude mouse assays, whereas the line MDA468 was not. Scatchard analysis indicated the presence of a single class of receptor for Tf on the rat and human mammary cell lines. The results suggest that neoplastic cells displaying various metastatic properties may express differing numbers of Tf receptors and respond differently to growth factors such as Tf. (C) 1993 Wiley-Liss, Inc.