A comparison of airborne ergosterol, glucan and Air-O-Cell data in relation to physical assessments of mold damage and some other parameters
A comparison of airborne ergosterol, glucan and Air-O-Cell data in relation to physical assessments of mold damage and some other parameters
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DOI：
10.1111/j.1600-0668.2005.00370.x
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发表时间：
2005-08-01
期刊：
影响因子：
5.8
通讯作者：
Dales, RE
中科院分区：
文献类型：
--
作者：
Foto, M;Vrijmoed, LLP;Dales, RE
我们在这里报告了 110 个家庭的长期空气样本的比较，其中对开放式过滤盒收集的材料进行了 β1,3-D 葡聚糖、麦角甾醇、胆固醇和内毒素的分析。然后将这些数据与仔细估计的可见霉菌和 Air-O-Cell 数据进行比较。除胆固醇外，发现的所有值均与有限数量的研究中报告的值相似。葡聚糖通过基于 G 因子的鲎变形细胞裂解物测定法以及尺寸排阻色谱法进行测量。这表明，在这些房屋中发现的大多数空气中的葡聚糖都是真菌来源的，来自酵母和完整的孢子，以及菌丝和孢子碎片。菌丝和孢子碎片合计占完整孢子数的 16%，但范围很广。空气中的葡聚糖与麦角甾醇和可见霉菌之间的相关性很强。然而，空气中的麦角甾醇与可见霉菌的相关性比葡聚糖更高。内毒素和 Air-O-Cell 测量值与研究中的其他测量值相关性较差或无关。这项研究使人们相信，长期空气样本中的毒素葡聚糖和麦角甾醇与建筑物损坏有关。
We report here a comparison of long duration air samples in 110 homes where the material collected on open faced filter cassettes was analyzed for beta 1,3-D glucan, ergosterol, cholesterol and endotoxin. These data were then compared to careful estimates of visible mold and Air-O-Cell data. All the values found except cholersterol were of a similar magnitude to values reported in the limited number of studies available. Glucan was measured with a factor G based assay of the Limulus Amebocyte Lysate followed by size exclusion chromatography. This showed that the majority of airborne glucan found in these houses was fungal in origin arising from both yeasts and intact spores, as well as hyphal and spore fragments. Hyphal and spore fragments together represented 16% of the intact spore counts but over a broad range. Correlations between airborne glucan were strong for ergosterol and visible mold. However, airborne ergosterol was more highly correlated to visible mold than glucan. Endotoxin and Air-O-Cell measurements were poorly or not related to the other measures in the study. This study provides confidence that long duration air samples of the toxin glucan and ergosterol are related to building damage.