A flow-activated chloride-selective membrane current in vascular endothelial cells
A flow-activated chloride-selective membrane current in vascular endothelial cells
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DOI：
10.1161/01.res.85.9.820
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发表时间：
1999-10-29
影响因子：
20.1
通讯作者：
Davies, PF
中科院分区：
文献类型：
--
作者：
Barakat, AI;Leaver, EV;Davies, PF
剪切应力诱导的内皮离子通道激活是目前最早的反应之一，涉及导致血管张力调节的机械信号转导。采用荧光电位染色法和全细胞膜片钳记录法研究了层流对内皮膜电位的影响。流动刺激膜超极化的应用，在35 ~ 160秒内逆转为去极化。脱极化是由独立于K+通道介导的超极化的流动激活的Cl-选择性膜电流引起的。因此，流动激活了K+和Cl-电流，净膜电位由响应的平衡决定。通过延长和排列细胞的流动预处理，膜电位对流动的敏感性不变。
Shear stress-induced activation of endothelial ion channels, one of the earliest responses to now, is implicated in mechano-signal transduction that results in the regulation of vascular tone. The effects of laminar flow on endothelial membrane potential were studied in vitro using both fluorescent potentiometric dye measurements and whole-cell patch-clamp recordings. The application of flow stimulated membrane hyperpolarization, which was reversed to depolarization within 35 to 160 seconds. The depolarization was caused by a Cl--selective membrane current activated by flow independently of the K+ channel-mediated hyperpolarization. Thus, flow activated both K+ and Cl- currents, with the net membrane potential being determined by the balance of the responses. Membrane potential sensitivity to flow was unchanged by flow preconditioning that elongated and aligned the cells.