Human Polyomavirus JC (JCV) Infection of Human B Lymphocytes: A Possible Mechanism for JCV Transmigration across the Blood-Brain Barrier
Human Polyomavirus JC (JCV) Infection of Human B Lymphocytes: A Possible Mechanism for JCV Transmigration across the Blood-Brain Barrier
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DOI：
10.1086/653823
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发表时间：
2010-07-15
影响因子：
6.4
通讯作者：
Nerurkar, Vivek R.
中科院分区：
文献类型：
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作者：
Chapagain, Moti L.;Nerurkar, Vivek R.
JC病毒（JC virus，JCV）可能通过感染的B细胞进入中枢神经系统。此外，最近的数据表明骨髓浆细胞中存在JCV。然而，JCV在B细胞中感染和复制的证据尚不清楚。为了解决这个问题，我们用JCV感染EB病毒转化的B细胞，发现病毒基因组从感染后第0天到第20天减少了>1000倍，这与病毒早期和晚期信使RNA转录物和蛋白质的缺乏同时发生。然而，用异硫氰酸荧光素缀合的JCV感染的B细胞的免疫荧光图像证明JCV进入B细胞，并且DNA酶保护试验证实了B细胞内存在完整的JCV病毒体。此外，JCV感染的B细胞能够将感染传递给幼稚神经胶质细胞。这些数据证实，JCV非生产性感染B细胞，并可能使用它们作为穿越血脑屏障的载体。
It has been suggested that JC virus (JCV) might travel to the central nervous system in infected B cells. Moreover, recent data suggest the presence of JCV in bone marrow plasma cells. However, the evidence for infection and replication of JCV in B cells is unclear. To address this question, we infected Epstein-Barr virus-transformed B cells with JCV and found that the viral genome decreased >1000-fold from days 0 to 20 after infection, which concurred with the absence of viral early and late messenger RNA transcripts and proteins. However, immunofluorescent images of B cells infected with fluorescein isothiocyanate-conjugated JCV demonstrated that JCV enters the B cells, and DNase protection assay confirmed the presence of intact JCV virions inside the B cells. Moreover, JCV-infected B cells were able to transmit infection to naive glial cells. These data confirm that JCV nonproductively infects B cells and possibly uses them as a vehicle for transmigration across the blood-brain barrier.