Take advantage of time in your experiments: a guide to simple, informative kinetics assays.
Take advantage of time in your experiments: a guide to simple, informative kinetics assays.
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利用您的实验时间：简单，有益的动力学测定指南。
DOI：
10.1091/mbc.e13-01-0030
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发表时间：
2013-04
影响因子：
3.3
通讯作者：
De La Cruz EM
中科院分区：
文献类型：
--
作者：
Pollard TD;De La Cruz EM
理解细胞和分子生物学中的几乎任何过程都取决于对生化反应速率的了解，因此遗憾的是很少有细胞和分子生物学家在他们的工作中利用动力学实验。幸运的是，对于理解细胞系统最有用的动力学实验对于每个研究将受益于这些信息的人来说都是触手可及的。本文描述了测量表征生命过程动力学的有价值的动力学参数的简单方法。这些“瞬态”方法不仅在概念上不同于用于分析稳态酶反应的传统方法，而且它们也适用于了解任何生物过程的动态。
Understanding virtually any process in cellular and molecular biology depends on knowledge of the rates of the biochemical reactions, so it is regrettable that few cellular and molecular biologists take advantage of kinetics experiments in their work. Fortunately, the kinetics experiments that are most useful for understanding cellular systems are within reach for everyone whose research would benefit from this information. This essay describes simple methods to measure the valuable kinetic parameters that characterize the dynamics of life processes. These “transient-state” methods not only differ in concept from traditional approaches used to analyze enzyme reactions at steady state, but they are also applicable to learning about the dynamics of any biological process.