An endophytic bacterium Acinetobacter calcoaceticus Sasm3-enhanced phytoremediation of nitrate-cadmium compound polluted soil by intercropping Sedum alfredii with oilseed rape
An endophytic bacterium Acinetobacter calcoaceticus Sasm3-enhanced phytoremediation of nitrate-cadmium compound polluted soil by intercropping Sedum alfredii with oilseed rape
复制标题
DOI：
10.1007/s11356-015-4933-5
复制
发表时间：
2015-11-01
影响因子：
5.8
通讯作者：
Yang, Xiaoe
中科院分区：
文献类型：
--
作者：
Chen, Bao;Ma, Xiaoxiao;Yang, Xiaoe
高投入的集约化农业生产方式是高产出的农业生产方式。然而，在集约化农业系统中，过度施肥有很大的潜力，造成土壤中硝酸盐和重金属的积累，对人体健康不利。研究了油菜间作和接种内生细菌Acinetobacter calcoaceticus Sasm 3对复合污染土壤的修复效果。结果表明，接种Sasm 3后，油菜地上部生物量增加10- 20%，根系增加64%，种子增加23- 29%，而根系硝酸盐积累量减少14%，地上部硝酸盐积累量减少12%。单作处理使油菜体内镉含量显著增加，间作处理使油菜体内镉含量显著降低。通过变性梯度凝胶电泳（DGGE）和实时荧光定量PCR分析，接种和/或间作处理显著增加了土壤细菌群落多样性和nirS、nirK基因拷贝数。可见，接种内生细菌Sasm 3的间作系统不仅提高了土壤镉的清除效率，而且在不影响作物生产的前提下，提高了作物的品质。
Intensive agricultural system with high input of fertilizer results in high agricultural output. However, excessive fertilization in intensive agricultural system has great potential to cause nitrate and heavy metal accumulation in soil, which is adverse to human health. The main objective of the present study was to observe the effects of intercropping and inoculation of endophytic bacterium Acinetobacter calcoaceticus Sasm3 on phytoremediation of combined contaminated soil in oilseed rape (Brassica napus L.). The results showed that with Sasm3 inoculation, the biomass of rape was increased by 10-20 % for shoot, 64 % for root, and 23-29 % for seeds while the nitrate accumulation in rape was decreased by 14 % in root and by 12 % in shoot. The cadmium concentration in rape increased significantly with mono-inoculating treatment, whereas it decreased significantly after intercropping treatment. By denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR analysis, the diversity of bacterial community and the number of nirS and nirK gene copies increased significantly with inoculation or/and intercropping treatment. In conclusion, the endophytic bacterium Sasm3-inoculated intercropping system not only improved the efficiency of clearing cadmium from soil without obstructing crop production, but also improved the quality of crop.