Polymerase chain reaction and restriction fragment length polymorphism analysis of varicella-zoster virus isolates from the United States and other parts of the world
Polymerase chain reaction and restriction fragment length polymorphism analysis of varicella-zoster virus isolates from the United States and other parts of the world
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DOI：
10.1086/514267
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发表时间：
1998-11-01
影响因子：
6.4
通讯作者：
Gershon, A
中科院分区：
文献类型：
--
作者：
LaRussa, P;Steinberg, S;Gershon, A
采用鉴别和区分水痘带状疱疹病毒（VZV）野生型（，vt）和疫苗株的聚合酶链反应（PCR）试验，确定日本境外wt合并液中是否存在限制性片段长度多态性与日本Oka疫苗株相似的VZV毒株。分析了来自美国和澳大利亚各地水痘患者和试验者的病毒样本（n = 114）。该测定正确鉴定了113个样品为野生型菌株。1份确定为Oka疫苗株的样本来自一名白血病儿童，该儿童在接种水痘减毒活疫苗后出现疫苗相关皮疹。在这一点上，没有证据表明类似疫苗的野生型菌株在日本以外地区传播。这表明该PCR测定可用于区分由于疫苗和野生型VZV引起的皮疹。
A polymerase chain reaction (PCR) assay that identifies and differentiates wild-type (,vt) and vaccine strains of varicella-zoster virus (VZV) was used to determine if VZV strains with restriction fragment length polymorphisms resembling those of the Japanese Oka vaccine strain were present in the wt pool outside of Japan. Virus samples (n = 114) from patients with chickenpox and tester from various parts of the United States and Australia were analyzed. The assay correctly identified 113 samples as wt strain. The 1 sample identified as Oka vaccine strain came from a child with leukemia who developed a vaccine-associated rash after receiving the live attenuated varicella vaccine. At this point, there is no evidence that wt strains resembling the vaccine are circulating outside of Japan. This indicates that this PCR assay can be utilized to distinguish rashes due to vaccine and wt VZV.