Peptide alignment of the porcine 21-hydroxylase cytochrome P-450 using a cDNA sequence of the corresponding bovine enzyme.
Peptide alignment of the porcine 21-hydroxylase cytochrome P-450 using a cDNA sequence of the corresponding bovine enzyme.
复制标题
使用相应牛酶的 cDNA 序列对猪 21-羟化酶细胞色素 P-450 进行肽比对。
DOI：
10.1016/0006-291x(84)90600-4
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Shively,JE
中科院分区：
文献类型：
--
作者：
Bienkowski,MJ;Haniu,M;Nakajin,S;Shinoda,M;Yanagibashi,K;Hall,PF;Shively,JE
从牛肾上腺类固醇21-羟化酶细胞色素P-450的cDNA克隆推导出的氨基酸序列已被用来比对来自相应的猪酶的肽序列。同源性分析表明，只有百分之五十的cDNA克隆预测的氨基酸序列与来自猪酶的肽序列重叠。通过考虑由cDNA序列的两个额外DNA阅读框预测的氨基酸序列，恢复了牛序列其余部分的同源性。由两个交替阅读框架预测的牛序列中有48%与猪肽序列具有很强的同源性。观察到的阅读框改变至少有4个核苷酸测序错误，并且已经确定了牛cDNA序列中每个错误的大致位置。
The amino acid sequence deduced from a cDNA clone of the bovine adrenal steroid 21-hydroxylase cytochrome P-450 has been utilized to align peptide sequences derived from the corresponding porcine enzyme. Homology analysis revealed that only fifty percent of the amino acid sequence predicted by the cDNA clone overlapped with peptide sequences from the porcine enzyme. The homology in the remaining portions of the bovine sequence was restored by considering amino acid sequences predicted by the two additional DNA reading frames of the cDNA sequence. Forty eight percent of the bovine sequences predicted by the two alternate reading frames showed strong homology with the porcine peptide sequences. A minimum of 4 nucleotide sequencing errors account for the observed reading frame alterations and the approximate position of each error in the bovine cDNA sequence has been established.