Volatile biomarkers of Pseudomonas aeruginosa in cystic fibrosis and noncystic fibrosis bronchiectasis
Volatile biomarkers of Pseudomonas aeruginosa in cystic fibrosis and noncystic fibrosis bronchiectasis
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DOI：
10.1111/j.1472-765x.2011.03049.x
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发表时间：
2011-06-01
影响因子：
2.4
通讯作者：
De Soyza, A.
中科院分区：
文献类型：
--
作者：
Savelev, S. U.;Perry, J. D.;De Soyza, A.
Aims：本研究的目的是确定是否可以在临床痰标本中检测到铜绿假单胞菌特异性的挥发性有机化合物。诊断Ps的金标准。铜绿假单胞菌感染者痰培养阳性。约72份痰液顶空样本取自有Ps风险或已知有Ps病史的患者。通过固相微萃取质谱分析铜绿假单胞菌感染。2-壬酮是Ps的标记物。痰液顶空气体检测铜绿假单胞菌的敏感性为72%，特异性为88%。挥发性化合物的组合，17种化合物与2-壬酮的痰库，增加了检测Ps的灵敏度。结论：与传统微生物培养48 h的周转期相比，本方法可在1-2 h内获得检测结果。这些数据证明了快速准确诊断Ps的潜力。研究的意义和影响：2-壬酮是需要在呼出气中进一步研究的化合物，因为它可以改善Ps的诊断。铜绿假单胞菌感染与其他报告的挥发性标志物相结合。
Aims:The purpose of this study was to determine whether volatile organic compounds specific to Pseudomonas aeruginosa could be detected in clinical sputum specimens.Methods and Results:Patients were recruited from specialist bronchiectasis and cystic fibrosis clinics. The gold standard for diagnosing Ps. aeruginosa infection was a positive sputum culture. About 72 sputum headspace samples taken from patients at risk of or known to have prior Ps. aeruginosa infection were analysed by solid phase micro-extraction mass spectrometry. 2-nonanone was a marker in Ps. aeruginosa in sputum headspace gas with sensitivity of 72% and specificity of 88%. A combination of volatile compounds, a sputum library of 17 compounds with 2-nonanone, increased sensitivity in the detection of Ps. aeruginosa to 91% with specificity of 88%.Conclusions:In contrast to the 48-hour turnaround for classical microbiological culture, these results were available within 1-2 h. These data demonstrate the potential for rapid and accurate diagnosis of Ps. aeruginosa infection from sputum samples.Significance and impact of the study:2-Nonanone is a compound requiring further study in the exhaled breath as it may improve diagnostic of Ps. aeruginosa infection when combined with other reported volatile markers.