Multiplex Reverse Transcription Polymerase Chain Reaction for Simultaneous Detection of Viruses in Gentian
Multiplex Reverse Transcription Polymerase Chain Reaction for Simultaneous Detection of Viruses in Gentian
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多重逆转录聚合酶链反应同时检测龙胆中的病毒
DOI：
10.1007/pl00013071
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发表时间：
2002
影响因子：
1.2
通讯作者：
Kazumi Suzuki
中科院分区：
文献类型：
--
作者：
T. Kuroda;Shoji Urushibara;I. Takeda;F. Nakatani;Kazumi Suzuki
采用多重反转录聚合酶链反应（RT-PCR）技术检测蚕豆枯萎病毒2型、黄瓜花叶病毒和三叶草黄脉病毒。该方法利用特异性引物对病毒的检测具有高度的敏感性和特异性，并根据扩增产物的大小对病毒种类进行鉴定。通过这种方法，还在病毒混合物中检测到三种病毒中的每一种。对247株龙胆进行多重RT-PCR检测，88%的大田植株感染了一种或多种病毒。
The multiplex reverse transcription polymerase chain reaction (RT-PCR) was used to detect Broad bean wilt virus 2, Cucumber mosaic virus and Clover yellow vein virus. Using specific primers, the method enabled detection of the viruses with a high degree of sensitivity and specificity and identification of the virus species according to the size of the PCR product amplified. Each of the three viruses was also detected within a mixture of the viruses by this method. Of 247 field plants of gentian subjected to the multiplex RT-PCR, 88% were found to be infected with one or more of the viruses.