An efficient CAPS marker for bacterial-blight resistance gene xa25 in rice
An efficient CAPS marker for bacterial-blight resistance gene xa25 in rice
复制标题
水稻白叶枯病抗性基因 xa25 的高效 CAPS 标记
DOI：
10.1080/07060661.2017.1336488
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发表时间：
2017
影响因子：
2
通讯作者：
Ma Bojun
中科院分区：
文献类型：
--
作者：
Zhang Yiting;Zhang Pinghua;Bhadauria Vijai;Chen Xifeng;Ma Bojun
摘要隐性黄单胞菌黄单胞菌致病型（Xanthomonasxanthropv.）水稻（Oryza sativa L.）中的Xoo抗性基因xa 25介导了对Xoo分离物PXO 339的小种特异性抗性。其显性等位基因Xa 25由Xoo感染转录诱导，这反过来又导致对PXO 339的易感性。利用xa 25/Xa 25启动子中的碱基替换（−40，G至T），设计了切割扩增多态性序列（CAPS）标记，用于有效鉴定xa 25基因。该标记与xa 25基因共分离，可明确区分水稻xa 25/Xa 25基因型。利用该标记对9个中国水稻地方品种进行检测，结果表明，xa 25/Xa 25基因型分别与水稻对PXO 339的抗性和敏感性相关。因此，该标记是可靠的和成本效益的标记辅助选择的水稻xa 25基因。
Abstract The recessive Xanthomonas oryzae pv. oryzae (Xoo) resistance gene, xa25, which mediates race-specific resistance to the Xoo isolate PXO339, has previously been characterized in rice (Oryza sativa L.). Its dominant allele, Xa25, is transcriptionally induced by Xoo infection, which in turn results in susceptibility to PXO339. Utilizing a base substitution (−40, G to T) in the xa25/Xa25 promoters, a cleaved amplified polymorphic sequence (CAPS) marker was designed for effective identification of the xa25 gene. This marker co-segregated with the xa25 gene and explicitly distinguished the genotypes of xa25/Xa25 gene in rice. The marker was further tested on nine Chinese rice landraces, revealing the association of xa25/Xa25 genotypes with resistance/susceptibility to PXO339, respectively. Therefore, this marker is reliable and cost-effective for marker-assisted selection of the xa25 gene in rice.