The FoxF/FoxC factor LET-381 directly regulates both cell fate specification and cell differentiation in C. elegans mesoderm development
The FoxF/FoxC factor LET-381 directly regulates both cell fate specification and cell differentiation in C. elegans mesoderm development
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DOI：
10.1242/dev.048496
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发表时间：
2010-05-01
期刊：
影响因子：
4.6
通讯作者：
Liu, Jun
中科院分区：
文献类型：
--
作者：
Amin, Nirav M.;Shi, Herong;Liu, Jun
叉头转录因子在中胚层发育过程中发挥着重要而多样的作用。特别是，FoxF和FoxC基因分别参与了腔体动物内脏/内脏中胚层和非内脏中胚层的发育。在这里，我们在单细胞分辨率下表明，在伪腔体线虫中，单一的FoxF/FoxC转录因子let-381在非肌肉中胚层细胞-体腔细胞(CCS)的指定和分化中以前馈机制发挥作用。LET-381/FoxF直接激活CC规范因子SIX2同源结构域蛋白CEH-34，并与CEH-34/SIX2协同作用，直接激活CC分化所需的基因。我们的结果统一了一系列关于FoxF/FoxC因子功能的不同研究，并为FoxF/FoxC因子在中胚层发育中的作用提供了一个模型。
Forkhead transcription factors play crucial and diverse roles in mesoderm development. In particular, FoxF and FoxC genes are, respectively, involved in the development of visceral/splanchnic mesoderm and non-visceral mesoderm in coelomate animals. Here, we show at single-cell resolution that, in the pseudocoelomate nematode C. elegans, the single FoxF/FoxC transcription factor LET-381 functions in a feed-forward mechanism in the specification and differentiation of the non-muscle mesodermal cells, the coelomocytes (CCs). LET-381/FoxF directly activates the CC specification factor, the Six2 homeodomain protein CEH-34, and functions cooperatively with CEH-34/Six2 to directly activate genes required for CC differentiation. Our results unify a diverse set of studies on the functions of FoxF/FoxC factors and provide a model for how FoxF/FoxC factors function during mesoderm development.