Antimicrobial activity of South African Podocarpus species
Antimicrobial activity of South African Podocarpus species
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DOI：
10.1016/j.jep.2008.06.023
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发表时间：
2008-09-02
影响因子：
5.4
通讯作者：
Van Staden, J.
中科院分区：
文献类型：
--
作者：
Abdillahi, H. S.;Stafford, G. I.;Van Staden, J.
民族药理学相关性：世界各地有几种罗汉松（罗汉松科）被用于治疗疾病。在非洲，四种罗汉松传统上用于动物和人类健康。研究目的：研究罗汉松属物种对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌、肺炎克雷伯菌和白色念珠菌的抗菌活性。材料和方法：使用六种不同极性的溶剂进行提取。使用微量稀释生物测定法评估抗菌活性，并使用微量稀释生物测定法和 (M27-P) 肉汤稀释液评估抗真菌活性。 结果：所有物种均表现出抗菌活性，MIC 值小于 1 mg/ml。对革兰氏阳性菌的抑制作用较强，MIC值为98μg/ml；对革兰氏阴性菌的抑制作用最强的是肺炎克雷伯菌，MIC值为0.33mg/ml。所有物种都表现出很强的抗真菌活性，48小时后最佳 MIC 为 30 μg/ml。结论：根据 Aligiannis 等人的研究，所有四个物种对所有测试的微生物都表现出强烈的抑制作用。 [Aligiannis, N.、Kalpotzakis, E.、Mitaku, S.、Chinou, I.B., 2001。两种牛至属植物精油的成分和抗菌活性。 Journal of Agriculture and Food Chemistry 40, 4168-4170]分类它们可被归类为强抑制剂。 (C) 2008 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Several species of Podocarpus (Podocarpaceae) are utilized in treating ailments across the world. In Africa, four species are used traditionally in both animal and human health.Aim of the study: To investigate the antimicrobial activity of Podocarpus species against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae and Candida albicans.Materials and methods: Six solvents of varying polarity were used for extraction. Antibacterial activity was assessed using the microdilution bioassay and for antifungal activity, the microdilution bioassay and (M27-P) broth dilution were used.Results: All species exhibited antimicrobial activity with MIC values of less than 1 mg/ml. Inhibition against Gram-positive bacteria was stronger with an MIC value of 98 mu g/ml while for Gram-negative bacteria, the highest inhibition was against Klebsiella pneumoniae with an MIC value of 0.33 mg/ml. All species exhibited strong antifungal activity with the best MIC being 30 mu g/ml after 48 h.Conclusions: All four species exhibited strong inhibition against all tested microbials, based on Aligiannis et al. [Aligiannis, N., Kalpotzakis, E., Mitaku, S., Chinou, I.B., 2001. Composition and antimicrobial activity of the essential oils of two Origanum species. journal of Agricultural and Food Chemistry 40, 4168-4170] classification they can be classified as strong inhibitors. (C) 2008 Elsevier Ireland Ltd. All rights reserved.