What can we learn from highly purified neutrophils?
What can we learn from highly purified neutrophils?
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DOI：
10.1042/bst0320468
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发表时间：
2004-06-01
影响因子：
3.9
通讯作者：
Whyte, MKB
中科院分区：
文献类型：
--
作者：
Sabroe, I;Prince, LR;Whyte, MKB
传统上，中性粒细胞纯化是通过密度梯度离心白细胞来进行的。这些可靠的方法产生的群体通常纯度 >95% 的中性粒细胞，并允许对这些细胞的功能进行广泛的研究。我们最近的工作表明，残留的单核细胞可能比以前意识到的发挥更重要的作用，并表明对于某些功能实验，需要进一步纯化细胞以充分了解中性粒细胞表型。
Neutrophil purification has traditionally been performed by centrifugation of leucocytes through density gradients. These reliable methods produce populations that are typically >95% pure neutrophils, and have allowed the widespread study of the function of these cells. our recent work has suggested that residual monocytes may play a more important role than has been previously realized, and suggest that for some functional experiments, further purification of cells is required to understand fully the neutrophil phenotype.