Identification of Tmem10/Opalin as a novel marker for oligodendrocytes using gene expression profiling
Identification of Tmem10/Opalin as a novel marker for oligodendrocytes using gene expression profiling
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DOI：
10.1186/1471-2202-9-40
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发表时间：
2008-04-25
期刊：
影响因子：
2.4
通讯作者：
Simons, Mikael
中科院分区：
文献类型：
--
作者：
Kippert, Angelika;Trajkovic, Katarina;Simons, Mikael
背景：在中枢神经系统的发育过程中，少突胶质细胞产生大量的髓磷脂，髓磷脂是一种包裹轴突的多层绝缘膜，从而允许动作电位的快速传导并保持轴突的完整性。少突胶质细胞向髓磷脂形成细胞的分化需要下调 RhoA GTPase 活性。结果：为了深入了解少突胶质细胞分化的分子机制，我们对用 Rho 激酶 (ROCK) 抑制剂 Y-27632 或用 条件神经元培养基。这导致了跨膜蛋白 10 (Tmem10/Opalin) 的鉴定，这是一种富含分化少突胶质细胞的新型 I 型跨膜蛋白。在原代培养物中，Tmem10 在 O4 阳性少突胶质细胞中大量表达，但在少突胶质细胞前体细胞、星形胶质细胞、小胶质细胞或神经元中不表达。在成熟的少突胶质细胞中，Tmem10 富集在细胞的边缘和突起中，仅在膜片中发现较少。结论： 总之，我们的结果表明 Tmem10 是体外生成的少突胶质细胞的新型标记物。
Background: During the development of the central nervous system, oligodendrocytes generate large amounts of myelin, a multilayered insulating membrane that ensheathes axons, thereby allowing the fast conduction of the action potential and maintaining axonal integrity. Differentiation of oligodendrocytes to myelin-forming cells requires the downregulation of RhoA GTPase activity.Results: To gain insights into the molecular mechanisms of oligodendrocyte differentiation, we performed microarray expression profiling of the oligodendroglial cell line, Oli-neu, treated with the Rho kinase ( ROCK) inhibitor, Y-27632 or with conditioned neuronal medium. This resulted in the identification of the transmembrane protein 10 ( Tmem10/Opalin), a novel type I transmembrane protein enriched in differentiating oligodendrocytes. In primary cultures, Tmem10 was abundantly expressed in O4-positive oligodendrocytes, but not in oligodendroglial precursor cells, astrocytes, microglia or neurons. In mature oligodendrocytes Tmem10 was enriched in the rims and processes of the cells and was only found to a lesser extent in the membrane sheets.Conclusion: Together, our results demonstrate that Tmem10 is a novel marker for in vitro generated oligodendrocytes.