High-throughput method for preliminary screening of high dietary fiber rice
High-throughput method for preliminary screening of high dietary fiber rice
复制标题
高通量方法初步筛选高膳食纤维大米
DOI：
10.1016/j.foodchem.2019.125192
复制
发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Shu Xiaoli
中科院分区：
文献类型：
--
作者：
Sun Jian;Zhang Ning;Ye Hongxia;Wu Dianxing;Shu Xiaoli
膳食纤维对人类有多种好处，开发具有改良膳食纤维成分的更健康大米已引起越来越多的关注。基于AOAC 2002.02的方法，我们开发了一种筛选高总膳食纤维（TDF）精米的简化方法。具有高TDF含量的突变体cw可以通过抗消化表型轻松地与R7954（籼稻）和Nipponbare（粳稻）区分开来，其特征是在胃蛋白酶、胰α-淀粉酶和淀粉葡萄糖苷酶水解后几乎完整的谷物。从F2群体中鉴定出具有消化抗性的个体，其TDF含量均高于5%，而那些不具有消化抗性表型的个体，其TDF含量低于5%。消化抗性表型可能是鉴定高TDF含量水稻的一个有价值的指标，该表型的鉴定为高TDF水稻育种提供了一种简单、经济、高通量的方法。
Dietary fiber has several benefits for humans, and the development of healthier rice with an improved dietary fiber composition has attracted increasing amounts of attention. Based on the method of AOAC 2002.02, we developed a simplified method to screen polished rice containing high total dietary fiber (TDF). Mutantcwwith a high TDF content could be distinguished easily from R7954 (indica) and Nipponbare (japonica) by the digestion-resistant phenotype, which is characterized as an almost intact grain after hydrolysis by pepsin, pancreatic α-amylase and amyloglucosidase. The individuals identified from the F2population showed digestion resistance all had TDF content higher than 5%, while those without a digestion-resistant phenotype had TDF content lower than 5%. The phenotype of digestion resistance could be a valuable index for identifying rice with higher TDF content, and the identification of this phenotype provides a simplified, economical and high throughput method for high TDF rice breeding.