Cancer and genomics
Cancer and genomics
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DOI：
10.1038/35057046
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发表时间：
2001-02-15
期刊：
影响因子：
64.8
通讯作者：
Stratton, MR
中科院分区：
文献类型：
--
作者：
Futreal, PA;Kasprzyk, A;Stratton, MR
鉴定导致肿瘤发生的基因是癌症研究的中心目标。我们搜索了从人类基因组序列草案中预测的蛋白质，以寻找已知肿瘤抑制基因的旁系同源物，但没有发现新的基因。然后，我们评估是否有可能通过比较癌症基因组序列与基因组草图直接搜索癌细胞中的致癌序列变化。显然，在正常组织和肿瘤组织中检测到相同程度的嵌合体转录物（来自染色体易位产生的致癌融合基因，其末端定位于不同的基因组位置），表明假阳性水平显著。我们的实验强调了目前可用的癌细胞DNA序列的有限数量和可变质量。
Identification of the genes that cause oncogenesis is a central aim of cancer research. We searched the proteins predicted from the draft human genome sequence for paralogues of known tumour suppressor genes, but no novel genes were identified. We then assessed whether it was possible to search directly for oncogenic sequence changes in cancer cells by comparing cancer genome sequences against the draft genome. Apparently chimaeric transcripts (from oncogenic fusion genes generated by chromosomal translocations, the ends of which mapped to different genomic locations) were detected to the same degree in both normal and neoplastic tissues, indicating a significant level of false positives. Our experiment underscores the limited amount and variable quality of DNA sequence from cancer cells that is currently available.