Inhibitory effects of Centella asiatica on azoxymethane-induced aberrant crypt focus formation and carcinogenesis in the intestines of F344 rats
Inhibitory effects of Centella asiatica on azoxymethane-induced aberrant crypt focus formation and carcinogenesis in the intestines of F344 rats
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DOI：
10.1016/j.fct.2004.06.022
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发表时间：
2004-12-01
影响因子：
4.3
通讯作者：
Ohnishi, Y
中科院分区：
文献类型：
--
作者：
Bunpo, P;Kataoka, K;Ohnishi, Y
积雪草水提物对小鼠抗抑郁作用的研究。本文观察了氧化偶氮甲烷（AOM）诱发的F344雄性大鼠异常隐窝灶（ACF）形成和小肠肿瘤发生的影响。与AOM处理组相比，用提取物处理显著减少了早期大肠中较大ACF（每个病灶有4个或更多隐窝）的数量，而甲基化DNA加合物的数量没有减少。在启动后阶段，提取物显着减少ACF的总数和较大的ACF的数量，伴随着5-溴-2 '-脱氧尿苷标记指数的降低和结肠粘膜中凋亡细胞的诱导增加。在以10 mg/kg的剂量喂食积雪草提取物的大鼠中，肿瘤的发生率、小肠和整个肠中的腺癌的数量以及整个肠中肿瘤的大小小于单独给予AOM的大鼠（p < 0.05）。100 mg/kg剂量的提取物显著降低了小肠中肿瘤的多样性（p < 0.05）。这些结果表明，抑制AOM诱导的ACF的形成，由C asiatica提取物与结肠隐窝中的细胞增殖和诱导细胞凋亡的修改，并且提取物对结肠肿瘤发生具有化学预防作用。(C)2004爱思唯尔有限公司保留所有权利。
Effects of the water extract of Centella asiatica Linn. on formation of azoxymethane (AOM)-induced aberrant crypt foci (ACF) and intestinal tumorigenesis in male F344 rats were investigated. Treatment with the extract significantly decreased the number of larger ACF (with four or more crypts per focus) in the large intestine in the early stage, while the number of methylated DNA adducts was not decreased compared with that in the AOM-treated group. In the post-initiation stage, the extract significantly decreased the total number of ACF and the number of larger ACF, accompanied by a decrease in the 5-bromo-2'-deoxyuridine-labeling index and an increase in the induction of apoptotic cells in the colonic mucosa. The incidences of neoplasms, the numbers of adenocarcinomas in the small intestines and entire intestines, and sizes of neoplasms in the entire intestines in rats fed C asiatica extract at a dose of 10 mg/kg were smaller than those in rats given AOM alone (p < 0.05). The extract at a dose of 100 mg/kg significantly reduced the multiplicity of neoplasms in the small intestine (p < 0.05). These results suggest that inhibition of the formation of AOM-induced ACF by C asiatica extract is associated with modification of cell proliferation and induction of apoptosis in colonic crypts and that the extract has a chemopreventive effect on colon tumorigenesis. (C) 2004 Elsevier Ltd. All rights reserved.