Disruption of the Salmonella-containing vacuole leads to increased replication of Salmonella enterica serovar typhimurium in the cytosol of epithelial cells
Disruption of the Salmonella-containing vacuole leads to increased replication of Salmonella enterica serovar typhimurium in the cytosol of epithelial cells
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DOI：
10.1128/iai.70.6.3264-3270.2002
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发表时间：
2002-06-01
影响因子：
3.1
通讯作者：
Finlay, BB
中科院分区：
文献类型：
--
作者：
Brumell, JH;Tang, P;Finlay, BB
鼠伤寒沙门氏菌（Salmonella enterica serovar Typhimurium）是一种兼性胞内病原体，其在受感染的宿主细胞中存在称为含沙门氏菌空泡（SCV）的空泡区室。SCV的维持是通过SifA完成的，并且这种沙门氏菌致病岛2型III型效应子的突变体在上皮细胞中更有效地复制。在这里，我们证明了sifA突变体的复制增强发生在这些细胞的胞质溶胶。在用渥曼青霉素或表达Rab5 Q79L或Rab7 N125I处理的细胞中也观察到野生型细菌的复制增加，所有这些都导致SCV完整性的丧失。我们的研究结果表明，需要宿主细胞内体系统的SCV的维护和损失，这间室允许增加复制血清型鼠伤寒在上皮细胞的胞质溶胶。
Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that inhabits a vacuolar compartment, called the Salmonella-containing vacuole (SCV), in infected host cells. Maintenance of the SCV is accomplished by SifA, and mutants of this Salmonella pathogenicity island 2 type III effector replicate more efficiently in epithelial cells. Here we demonstrate that enhanced replication of sifA mutants occurs in the cytosol of these cells. Increased replication of wild-type bacteria was also observed in cells treated with wortmannin or expressing Rab5 Q79L or Rab7 N125I, all of which caused a loss of SCV integrity. Our findings demonstrate the requirement of the host cell endosomal system for maintenance of the SCV and that loss of this compartment allows increased replication of serovar Typhimurium in the cytosol of epithelial cells.