Lens-specific VEGF-A expression induces angioblast migration and proliferation and stimulates angiogenic remodeling
Lens-specific VEGF-A expression induces angioblast migration and proliferation and stimulates angiogenic remodeling
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DOI：
10.1006/dbio.2000.9755
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发表时间：
2000-07-15
影响因子：
2.7
通讯作者：
Overbeek, PA
中科院分区：
文献类型：
--
作者：
Ash, JD;Overbeek, PA
血管内皮生长因子（VEGF）是一种分泌型促有丝分裂原，在体内外特异性刺激血管内皮细胞增殖。其表达模式与其作为血管发生和血管生成的重要调节剂一致，并且VEGF-A的靶向破坏已证明其对于血管发育是必不可少的。为了确定VEGF-A是否足以改变眼睛中的血管形成，我们产生了在透镜中特异性表达人VEGF-A（165）的转基因小鼠。转基因VEGF-A的表达导致透镜周围成血管细胞和内皮细胞的过度增殖和聚集。结果支持VEGF-A可以通过刺激成血管细胞和内皮细胞的化学吸引和增殖来启动血管化过程以及VEGF-A表达可以刺激血管生成重构的假设。然而，单独的VEGF-A不足以指导血管组织化或成熟，（C）2000学术出版社。
Vascular endothelial growth factor (VEGF) is a secreted mitogen which specifically stimulates proliferation of vascular endothelial cells in vitro and in vivo. Its expression pattern is consistent with it being an important regulator of vasculogenesis and angiogenesis, and targeted disruption of VEGF-A has demonstrated that it is essential for vascular development. To determine if VEGF-A was sufficient to alter vascularization in the eye we generated transgenic mice which express human VEGF-A(165) specifically in the lens. Expression of transgenic VEGF-A led to excessive proliferation and accumulation of disorganized angioblasts and endothelial cells around the lens. The results support the hypothesis that VEGF-A can initiate the process of vascularization by stimulating chemoattraction and proliferation of angioblasts and endothelial cells and that VEGF-A expression can stimulate angiogenic remodeling. However, VEGF-A alone was not sufficient to direct blood vessel organization or maturation, (C) 2000 Academic Press.