Phenotype and genotype of pancreatic cancer cell lines.
Phenotype and genotype of pancreatic cancer cell lines.
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DOI：
10.1097/mpa.0b013e3181c15963
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发表时间：
2010-05
期刊：
影响因子：
2.9
通讯作者：
Mulvihill SJ
中科院分区：
文献类型：
--
作者：
Deer EL;González-Hernández J;Coursen JD;Shea JE;Ngatia J;Scaife CL;Firpo MA;Mulvihill SJ
胰腺癌（PA）的预后不佳部分是由于缺乏有关疾病发展的分子信息。已建立的细胞系仍然是研究这些分子事件的有用工具。在这里，我们提出了一个常用的PA细胞系作为资源，以帮助研究人员选择最适合他们的特定研究需要的细胞系的可用信息的审查。评价了临床病史、体外和体内生长特征、表型特征（如粘附、侵袭、迁移和肿瘤发生）以及常见改变基因（KRAS、p53、p16和SMAD 4）的基因型状态等信息。文献中一致和不一致信息的识别表明，在选择细胞系之前应进行仔细评估，并注意细胞系认证。
The dismal prognosis of pancreatic adenocarcinoma (PA) is due in part due to a lack of molecular information regarding disease development. Established cell lines remain a useful tool for investigating these molecular events. Here we present a review of available information on commonly used PA cell lines as a resource to help investigators select the cell lines most appropriate for their particular research needs. Information on clinical history, in vitro and in vivo growth characteristics, phenotypic characteristics, such as adhesion, invasion, migration and tumorigenesis, and genotypic status of commonly altered genes (KRAS, p53, p16, and SMAD4) was evaluated. Identification of both consensus and discrepant information in the literature suggests careful evaluation before selection of cell lines and attention be given to cell line authentication.